Mass-tag technology for monitoring of protein kinase activity using mass spectrometry

被引：12

作者：

Sonoda, T

Shigaki, S

Nagashima, T

Okitsu, O

Kita, Y

Murata, M

Katayama, Y ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch Engn, Dept Appl Chem, Higashi Ku, Fukuoka 8128581, Japan

[2] Fujisawa Pharmaceut Co Ltd, Adv Technol Platform Lab, Tsukuba, Ibaraki 3002698, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2004年 / 14卷 / 04期

关键词：

protein kinases; protein phosphorylation; high-throughput screening; mass spectrometry;

D O I：

10.1016/j.bmcl.2003.12.022

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Monitoring of intracellular protein kinase activity is very important for fields involving diagnosis and drug screening. However, current methods, such as radiometry using P-32, or ELISA, are laborious and time-consuming. We have developed high-throughput assay system of protein kinase activity using mass-tagged substrate peptide probes and mass spectrometry. This assay system can easily evaluate target kinase activity and will potentially be able to simultaneously profile many protein kinase activities. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：847 / 850

页数：4

共 15 条

[1] Microphthalmia gene product as a signal transducer in cAMP-induced differentiation of melanocytes
Bertolotto, C
Abbe, P
Hemesath, TJ
Bille, K
Fisher, DE
Ortonne, JP
Ballotti, R
[J]. JOURNAL OF CELL BIOLOGY, 1998, 142 (03) : 827 - 835
[2] Antioxidant-induced nuclear translocation of CCAAT/enhancer-binding protein beta - A critical role for protein kinase A-mediated phosphorylation of Ser(299)
Chinery, R
Brockman, JA
Dransfield, DT
Coffey, RJ
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (48) : 30356 - 30361
[3] ADENOSINE CYCLIC 3',5'-MONOPHOSPHATE DEPENDENT PROTEIN-KINASE - KINETIC MECHANISM FOR THE BOVINE SKELETAL-MUSCLE CATALYTIC SUBUNIT
COOK, PF
NEVILLE, ME
VRANA, KE
HARTL, FT
ROSKOSKI, R
[J]. BIOCHEMISTRY, 1982, 21 (23) : 5794 - 5799
[4] Caspase-3: A vulnerability factor and final effector in apoptotic death of dopaminergic neurons in Parkinson's disease
Hartmann, A
Hunot, S
Michel, PP
Muriel, MP
Vyas, S
Faucheux, BA
Mouatt-Prigent, A
Turmel, H
Srinivasan, A
Ruberg, M
Evan, GI
Agid, Y
Hirsch, EC
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (06) : 2875 - 2880
[5] Bmk1/Erk5 is required for cell proliferation induced by epidermal growth factor
Kato, Y
Tapping, RI
Huang, S
Watson, MH
Ulevitch, RJ
Lee, JD
[J]. NATURE, 1998, 395 (6703) : 713 - 716
[6] Human breast cancer cells contain elevated levels and activity of the protein kinase, PKR
Kim, SH
Forman, AP
Mathews, MB
Gunnery, S
[J]. ONCOGENE, 2000, 19 (27) : 3086 - 3094
[7] A chemiluminescent microtiter plate assay for sensitive detection of protein kinase activity
Lehel, C
DanielIssakani, S
Brasseur, M
Strulovici, B
[J]. ANALYTICAL BIOCHEMISTRY, 1997, 244 (02) : 340 - 346
[8] McCusker RH, 1998, J CELL PHYSIOL, V174, P293, DOI 10.1002/(SICI)1097-4652(199803)174:3<293::AID-JCP3>3.0.CO
[9] 2-J
[10] Transcriptional regulation by cyclic AMP
Montminy, M
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1997, 66 : 807 - 822

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