Sensitivity of nested PCR in the detection of low numbers of Giardia lamblia cysts

被引：19

作者：

Miller, Kathryn M. ^{[1
]}

Sterling, Charles R. ^{[1
]}

机构：

[1] Univ Arizona, Dept Vet Sci & Microbiol, Tucson, AZ 85721 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 18期

关键词：

D O I：

10.1128/AEM.00668-07

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Nested PCR was performed on individually isolated Giardia lamblia cysts in replicates of 50 for sets of 1, 2, 3, 4, 5, 7, and 10 cysts. Amplification ranged from 80% for 1 cyst to 100% for 10 cysts. The results suggest that nested PCR is well adapted for G. lamblia single-cyst detection.

引用

页码：5949 / 5950

页数：2

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