A cis-acting regulatory element that affects the alternative splicing of a muscle-specific exon in the mouse NCAM gene

被引：10

作者：

Kawahigashi, H ^{[1
]}

Harada, Y ^{[1
]}

Asano, A ^{[1
]}

Nakamura, M ^{[1
]}

机构：

[1] Osaka Univ, Inst Prot Res, Suita, Osaka 565, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1998年 / 1397卷 / 03期

关键词：

NCAM; alternative splicing; myoblast; fibroblast; differentiation; MyoD;

D O I：

10.1016/S0167-4781(98)00021-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The pre-mRNA encoding the neural cell adhesion molecule (NCAM) is spliced to generate NCAM isoforms containing the muscle-specific domain (MSD) during myogenesis. Utilizing chimeric NCAM minigenes, we searched for cis-acting elements that contribute to the alternative selection of exon MSDb, one of the four exons encoding MSD, and identified an intronic cis-element located downstream of exon MSDb. The cis-element acted as a negative regulator for the selection of exon MSDb in nonmuscle fibroblasts but not in myoblasts, that are already destined to differentiate into muscle cells. The suppressive effect of this cis-element on the selection of exon MSDb was released in the process of myogenesis. When MyoD was co-expressed with a minigene containing this element in fibroblasts, the suppressive effect of the cis-element was released as the cells underwent differentiation. We propose that this cis-element contributes at least as one of the regulatory elements in the differentiation state-dependent selection of MSD exons in vivo. (C) 1998 Elsevier Science B.V.

引用

页码：305 / 315

页数：11