Derangement of the T-cell repertoire in patients with B-cell non-Hodgkin's lymphoma

被引：14

作者：

Fozza, Claudio ^{[1
]}

Corda, Giovanna ^{[1
]}

Virdis, Patrizia ^{[1
]}

Contini, Salvatore ^{[1
]}

Barraqueddu, Francesca ^{[1
]}

Galleu, Antonio ^{[1
]}

Isoni, Antonella ^{[1
]}

Cossu, Antonella ^{[1
]}

Dore, Fausto ^{[1
]}

Careddu, Maria G. ^{[1
]}

Bonfigli, Silvana ^{[1
]}

Giannico, Barbara ^{[1
]}

Longinotti, Maurizio ^{[1
]}

机构：

[1] Univ Sassari, Dept Biomed Sci, I-07100 Sassari, Italy

来源：

EUROPEAN JOURNAL OF HAEMATOLOGY | 2015年 / 94卷 / 04期

关键词：

non-Hodgkin's lymphoma; T-cell receptor repertoire; regulatory T cells; flow cytometry; spectratyping; third complementarity determining region; QUALITY-OF-LIFE; MYELODYSPLASTIC SYNDROMES; FOLLICULAR LYMPHOMA; FOLLOW-UP; CD4(+); PATHOGENESIS; COMPLEXITY; EXPRESSION; SUBSETS; DISEASE;

D O I：

10.1111/ejh.12417

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Although a number of studies suggest that different immune pathways may play a role in the pathogenesis of non-Hodgkin's lymphomas (NHL), the shape of the T-cell compartment has been only superficially explored in these patients. In our study, we analyzed the peripheral T-cell receptor (TCR) repertoire and the distribution of different T-cell subsets - including regulatory T cells (Treg) - in 30 patients with NHL, by combining flow cytometry and spectratyping. We first demonstrated by flow cytometry an increased frequency of expanded T-cell subpopulations expressing the same TCR beta variable (BV) subfamilies in CD8+ cells from NHL patients when compared with healthy controls, beside a higher frequency of Treg. Moreover, NHL patients were characterized by a higher percentage of BVs showing a skewed CDR3 profile both in CD4+ and CD8+ cells when analyzed by spectratyping. Our data suggest that the T-cell branch of the immune system of patients with B-cell NHL is deeply deranged, as witnessed by the increased degree of activation and skewing of their TCR repertoire along with the higher frequency of Treg.

引用

页码：298 / 309

页数：12

共 36 条

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