Influence of shear stress and size on viability of endothelial cells exposed to gold nanoparticles
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作者:
Fede, C.
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Univ Padua, Ist Anat, Dipartimento Neurosci, Via Gabelli 65, I-35131 Padua, ItalyUniv Padua, Ist Anat, Dipartimento Neurosci, Via Gabelli 65, I-35131 Padua, Italy
Fede, C.
[1
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Albertin, Giovanna
[1
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Petrelli, L.
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Univ Padua, Ist Anat, Dipartimento Neurosci, Via Gabelli 65, I-35131 Padua, ItalyUniv Padua, Ist Anat, Dipartimento Neurosci, Via Gabelli 65, I-35131 Padua, Italy
Petrelli, L.
[1
]
De Caro, R.
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Univ Padua, Ist Anat, Dipartimento Neurosci, Via Gabelli 65, I-35131 Padua, ItalyUniv Padua, Ist Anat, Dipartimento Neurosci, Via Gabelli 65, I-35131 Padua, Italy
De Caro, R.
[1
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Fortunati, I.
[2
,3
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Weber, V.
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机构:
Univ Padua, Dipartimento Sci Chim, Via Marzolo 1, I-35131 Padua, Italy
Univ Padua, UdR INSTM, Via Marzolo 1, I-35131 Padua, ItalyUniv Padua, Ist Anat, Dipartimento Neurosci, Via Gabelli 65, I-35131 Padua, Italy
Weber, V.
[2
,3
]
Ferrante, Camilla
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Univ Padua, Dipartimento Sci Chim, Via Marzolo 1, I-35131 Padua, Italy
Univ Padua, UdR INSTM, Via Marzolo 1, I-35131 Padua, ItalyUniv Padua, Ist Anat, Dipartimento Neurosci, Via Gabelli 65, I-35131 Padua, Italy
Ferrante, Camilla
[2
,3
]
机构:
[1] Univ Padua, Ist Anat, Dipartimento Neurosci, Via Gabelli 65, I-35131 Padua, Italy
[2] Univ Padua, Dipartimento Sci Chim, Via Marzolo 1, I-35131 Padua, Italy
Screening nanoparticle toxicity directly on cell culture can be a fast and cheap technique. Nevertheless, to obtain results in accordance with those observed in live animals, the conditions in which cells are cultivated should resemble the one encountered in live systems. Microfluidic devices offer the possibility to satisfy this requirement, in particular with endothelial cell lines, because they are capable to reproduce the flowing media and shear stress experienced by these cell lines in vivo. In this work, we exploit a microfluidic device to observe how human umbilical vein endothelial cells (HUVEC) viability changes when subject to a continuous flow of culture medium, in which spherical citrate-stabilized gold nanoparticles of different sizes and at varying doses are investigated. For comparison, the same experiments are also run in multiwells where the cells do not experience the shear stress induced by the flowing medium. We discuss the results considering the influence of mode of exposure and nanoparticle size (24 and 13 nm). We observed that gold nanoparticles show a lower toxicity under flow conditions with respect to static and the HUVEC viability decreases as the nanoparticle surface area per unit volume increases, regardless of size.
机构:
US Army, Corps Engineers, Engineer Res & Dev Ctr, Environm Lab, Vicksburg, MS 39180 USAUS Army, Corps Engineers, Engineer Res & Dev Ctr, Environm Lab, Vicksburg, MS 39180 USA
Cuddy, Michael F.
Poda, Aimee R.
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机构:
US Army, Corps Engineers, Engineer Res & Dev Ctr, Environm Lab, Vicksburg, MS 39180 USAUS Army, Corps Engineers, Engineer Res & Dev Ctr, Environm Lab, Vicksburg, MS 39180 USA
Poda, Aimee R.
Moser, Robert D.
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机构:
US Army, Corps Engineers, Engineer Res & Dev Ctr, Geotech & Struct Lab, Vicksburg, MS 39180 USAUS Army, Corps Engineers, Engineer Res & Dev Ctr, Environm Lab, Vicksburg, MS 39180 USA
Moser, Robert D.
Weiss, Charles A.
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h-index: 0
机构:
US Army, Corps Engineers, Engineer Res & Dev Ctr, Geotech & Struct Lab, Vicksburg, MS 39180 USAUS Army, Corps Engineers, Engineer Res & Dev Ctr, Environm Lab, Vicksburg, MS 39180 USA
Weiss, Charles A.
Cairns, Carolyn
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机构:US Army, Corps Engineers, Engineer Res & Dev Ctr, Environm Lab, Vicksburg, MS 39180 USA
Cairns, Carolyn
Steevens, Jeffery A.
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h-index: 0
机构:
US Army, Corps Engineers, Engineer Res & Dev Ctr, Environm Lab, Vicksburg, MS 39180 USAUS Army, Corps Engineers, Engineer Res & Dev Ctr, Environm Lab, Vicksburg, MS 39180 USA
机构:
US Army, Corps Engineers, Engineer Res & Dev Ctr, Environm Lab, Vicksburg, MS 39180 USAUS Army, Corps Engineers, Engineer Res & Dev Ctr, Environm Lab, Vicksburg, MS 39180 USA
Cuddy, Michael F.
Poda, Aimee R.
论文数: 0引用数: 0
h-index: 0
机构:
US Army, Corps Engineers, Engineer Res & Dev Ctr, Environm Lab, Vicksburg, MS 39180 USAUS Army, Corps Engineers, Engineer Res & Dev Ctr, Environm Lab, Vicksburg, MS 39180 USA
Poda, Aimee R.
Moser, Robert D.
论文数: 0引用数: 0
h-index: 0
机构:
US Army, Corps Engineers, Engineer Res & Dev Ctr, Geotech & Struct Lab, Vicksburg, MS 39180 USAUS Army, Corps Engineers, Engineer Res & Dev Ctr, Environm Lab, Vicksburg, MS 39180 USA
Moser, Robert D.
Weiss, Charles A.
论文数: 0引用数: 0
h-index: 0
机构:
US Army, Corps Engineers, Engineer Res & Dev Ctr, Geotech & Struct Lab, Vicksburg, MS 39180 USAUS Army, Corps Engineers, Engineer Res & Dev Ctr, Environm Lab, Vicksburg, MS 39180 USA
Weiss, Charles A.
Cairns, Carolyn
论文数: 0引用数: 0
h-index: 0
机构:US Army, Corps Engineers, Engineer Res & Dev Ctr, Environm Lab, Vicksburg, MS 39180 USA
Cairns, Carolyn
Steevens, Jeffery A.
论文数: 0引用数: 0
h-index: 0
机构:
US Army, Corps Engineers, Engineer Res & Dev Ctr, Environm Lab, Vicksburg, MS 39180 USAUS Army, Corps Engineers, Engineer Res & Dev Ctr, Environm Lab, Vicksburg, MS 39180 USA