Introduction to Single-Cell DNA Methylation Profiling Methods

被引：39
|
作者：
Ahn, Jongseong [1 ]
Heo, Sunghoon [1 ]
Lee, Jihyun [2 ,3 ]
Bang, Duhee [1 ]
机构：
[1] Yonsei Univ, Dept Chem, Seoul 03722, South Korea
[2] Kyung Hee Univ, Coll Med, Dept Clin Pharmacol & Therapeut, Seoul 02447, South Korea
[3] Kyung Hee Univ, Dept Biomed Sci & Technol, Seoul 02447, South Korea
来源：
BIOMOLECULES | 2021年 / 11卷 / 07期
基金：
新加坡国家研究基金会;
关键词：
DNA methylation; single cell; bioinformatics; GENOME-WIDE; METHYLOME ANALYSIS; CPG ISLANDS; RNA-SEQ; IDENTIFICATION; CANCER; LANDSCAPES; EPIGENOME; CAPTURE; INPUT;
D O I：
10.3390/biom11071013
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
DNA methylation is an epigenetic mechanism that is related to mammalian cellular differentiation, gene expression regulation, and disease. In several studies, DNA methylation has been identified as an effective marker to identify differences between cells. In this review, we introduce single-cell DNA-methylation profiling methods, including experimental strategies and approaches to computational data analysis. Furthermore, the blind spots of the basic analysis and recent alternatives are briefly described. In addition, we introduce well-known applications and discuss future development.
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页数：20
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