Expressed sequence tags from persimmon at different developmental stages

被引:31
作者
Nakagawa, T. [2 ]
Nakatsuka, A. [1 ]
Yano, K. [3 ]
Yasugahira, S. [1 ]
Nakamura, R. [1 ]
Sun, N. [1 ]
Itai, A. [4 ]
Suzuki, T. [5 ]
Itamura, H. [1 ]
机构
[1] Shimane Univ, Fac Life & Environm Sci, Matsue, Shimane 6908504, Japan
[2] Shimane Univ, Ctr Integrated Res Sci, Dept Mol & Funct Genom, Matsue, Shimane 6908504, Japan
[3] Univ Tokyo, Grad Sch Agr & Life Sci, Tokyo 1138657, Japan
[4] Tottori Univ, Fac Agr, Tottori 6808533, Japan
[5] Nagoya Univ, Grad Sch Sci, Nagoya, Aichi 4648601, Japan
关键词
carotenoid; Diospyros kaki; fruit; proanthocyanidin; ripening;
D O I
10.1007/s00299-008-0518-9
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Persimmon (Diospyros kaki Thunb.) is an important fruit in Asian countries, where it is eaten as a fresh fruit and is also used for many other purposes. To understand the molecular mechanism of fruit development and ripening in persimmon, we generated a total of 9,952 expressed sequence tags (ESTs) from randomly selected clones of two different cDNA libraries. One cDNA library was derived from fruit of "Saijo" persimmon at an early stage of development, and the other from ripening fruit. These ESTs were clustered into 6,700 non-redundant sequences. Of the 6,700 non-redundant sequences evaluated, the deduced amino acid sequences of 4,356 (65%) showed significant homology to known proteins, and 2,344 (35%) showed no significant similarity to any known proteins in Arabidopsis databases. We report comparison of genes identified in the two cDNA libraries and describe some putative genes involved in proanthocyanidin and carotenoid synthesis. This study provides the first global overview of a set of genes that are expressed during fruit development and ripening in persimmon.
引用
收藏
页码:931 / 938
页数:8
相关论文
共 40 条
[1]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[2]   Functional annotation of the Arabidopsis genome using controlled vocabularies [J].
Berardini, TZ ;
Mundodi, S ;
Reiser, L ;
Huala, E ;
Garcia-Hernandez, M ;
Zhang, PF ;
Mueller, LA ;
Yoon, J ;
Doyle, A ;
Lander, G ;
Moseyko, N ;
Yoo, D ;
Xu, I ;
Zoeckler, B ;
Montoya, M ;
Miller, N ;
Weems, D ;
Rhee, SY .
PLANT PHYSIOLOGY, 2004, 135 (02) :745-755
[3]   Kiwi protein inhibitor of pectin methylesterase - Amino-acid sequence and structural importance of two disulfide bridges [J].
Camardella, L ;
Carratore, V ;
Ciardiello, MA ;
Servillo, L ;
Balestrieri, C ;
Giovane, A .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (14) :4561-4565
[4]   The in vitro inhibitory effect of tannin derivatives on 3-hydroxy-3-methylglutaryl-coenzyme A reductase on Vero cells [J].
Chang, JJ ;
Chen, TH ;
Chan, P ;
Chen, YJ ;
Hsu, FL ;
Lo, MY ;
Lin, JY .
PHARMACOLOGY, 2001, 62 (04) :224-228
[5]  
Chang S. J., 1993, Plant Molecular Biology Reporter, V11, P113, DOI 10.1007/BF02670468
[6]   Interaction between the tobacco mosaic virus movement protein and host cell pectin methylesterases is required for viral cell-to-cell movement [J].
Chen, MH ;
Sheng, JS ;
Hind, G ;
Handa, AK ;
Citovsky, V .
EMBO JOURNAL, 2000, 19 (05) :913-920
[7]   Characterizing the grape transcriptome. Analysis of expressed sequence tags from multiple vitis species and development of a compendium of gene expression during berry development [J].
da Silva, FG ;
Iandolino, A ;
Al-Kayal, F ;
Bohlmann, MC ;
Cushman, MA ;
Lim, H ;
Ergul, A ;
Figueroa, R ;
Kabuloglu, EK ;
Osborne, C ;
Rowe, J ;
Tattersall, E ;
Leslie, A ;
Xu, J ;
Baek, J ;
Cramer, GR ;
Cushman, JC ;
Cook, DR .
PLANT PHYSIOLOGY, 2005, 139 (02) :574-597
[8]   Discovery of genes associated with fruit ripening in Carica papaya using expressed sequence tags [J].
Devitt, LC ;
Sawbridge, T ;
Holton, TA ;
Mitchelson, K ;
Dietzgen, RG .
PLANT SCIENCE, 2006, 170 (02) :356-363
[9]   Base-calling of automated sequencer traces using phred.: I.: Accuracy assessment [J].
Ewing, B ;
Hillier, L ;
Wendl, MC ;
Green, P .
GENOME RESEARCH, 1998, 8 (03) :175-185
[10]   Comprehensive EST analysis of tomato and comparative genomics of fruit ripening [J].
Fei, ZJ ;
Tang, X ;
Alba, RM ;
White, JA ;
Ronning, CM ;
Martin, GB ;
Tanksley, SD ;
Giovannoni, JJ .
PLANT JOURNAL, 2004, 40 (01) :47-59