A Tryptophan-Rich Motif in the Human Parainfluenza Virus Type 2 V Protein Is Critical for the Blockade of Toll-Like Receptor 7 (TLR7)- and TLR9-Dependent Signaling

被引：29

作者：

Kitagawa, Yoshinori ^{[1
]}

Yamaguchi, Mayu ^{[1
,2
]}

Zhou, Min ^{[1
,2
]}

Komatsu, Takayuki ^{[3
]}

Nishio, Machiko ^{[4
]}

Sugiyama, Tsuyoshi ^{[5
]}

Takeuchi, Kenji ^{[6
]}

Itoh, Masae ^{[2
]}

Gotoh, Bin ^{[1
]}

机构：

[1] Shiga Univ Med Sci, Div Microbiol & Infect Dis, Dept Pathol, Shiga 5202192, Japan

[2] Nagahama Inst Biosci & Technol, Fac Biosci, Shiga 5260829, Japan

[3] Aichi Med Univ, Dept Microbiol & Immunol, Sch Med, Aichi 4801195, Japan

[4] Mie Univ, Dept Microbiol, Grad Sch Med, Tsu, Mie 5148507, Japan

[5] Gifu Pharmaceut Univ, Microbiol Lab, Dept Publ Hlth Pharm, Gifu 5028585, Japan

[6] Univ Fukui, Microbiol Sect, Dept Pathol Sci, Fac Med Sci, Eiheiji, Fukui 9101193, Japan

来源：

JOURNAL OF VIROLOGY | 2011年 / 85卷 / 09期

基金：

日本学术振兴会;

关键词：

PLASMACYTOID DENDRITIC CELLS; INTERFERON EVASION; ALPHA; IRF-7; EXPRESSION; INFECTION; PATHWAY; MYD88; RNA;

D O I：

10.1128/JVI.02012-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Plasmacytoid dendritic cells (pDCs) do not produce alpha interferon (IFN-alpha) unless viruses cause a systemic infection or overcome the first-line defense provided by conventional DCs and macrophages. We show here that even paramyxoviruses, whose infections are restricted to the respiratory tract, have a V protein able to prevent Toll-like receptor 7 (TLR7)- and TLR9-dependent IFN-alpha induction specific to pDCs. Mutational analysis of human parainfluenza virus type 2 demonstrates that the second Trp residue of the Trp-rich motif (Trp-X-3-Trp-X-9-Trp) in the C-terminal domain unique to V, a determinant for IRF7 binding, is critical for the blockade of TLR7/9-dependent signaling.

引用

页码：4606 / 4611

页数：6

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