Identification of microorganisms grown in blood culture flasks using liquid chromatography-tandem mass spectrometry

被引：13

作者：

Berendsen, Erwin M. ^{[1
]}

Levin, Evgeni ^{[2
]}

Braakman, Rene ^{[1
]}

van der Riet-van Oeveren, Debora ^{[1
]}

Sedee, Norbert J. A. ^{[1
]}

Paauw, Armand ^{[1
]}

机构：

[1] Netherlands Org Appl Sci Res TNO, Dept CBRN Protect, Lange Kleiweg 137, NL-2288 GJ Rijswijk, Netherlands

[2] Netherlands Org Appl Sci Res TNO, Dept Microbiol & Syst Biol, Utrechtseweg 48, NL-3704 HE Zeist, Netherlands

来源：

FUTURE MICROBIOLOGY | 2017年 / 12卷 / 13期

关键词：

bloodstream infection; identification; LC-MS/MS; DESORPTION IONIZATION-TIME; LASER-DESORPTION/IONIZATION-TIME; ESCHERICHIA-COLI; HIGH-RESOLUTION; BACTERIA; INFECTIONS; SYSTEM; RESISTANCE; EVOLUTION; DIAGNOSIS;

D O I：

10.2217/fmb-2017-0050

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Aim: Bloodstream infections are a common cause of disease and a fast and accurate identification of the causative agent or agents of bloodstream infections would aid the start of adequate treatment. Materials & methods: A liquid chromatography-tandem mass spectrometry (LC-MS/MS) shotgun proteomics method was developed for the identification of bacterial species directly from blood cultures that were simulated by inoculating blood culture bottles with single or multiple clinically relevant microorganisms. Results: Using LC-MS/MS, the single species were correctly identified in 100% of the blood cultures, whereas for polymicrobial infections, 78% of both species were correctly identified in blood cultures. Conclusion: The LC-MS/MS method allows for the identification of the causative agent of positive blood cultures.

引用

页码：1135 / 1145

页数：11

共 36 条

[1] Secular Trends in Nosocomial Bloodstream Infections: Antibiotic-Resistant Bacteria Increase the Total Burden of Infection [J].

Ammerlaan, H. S. M. ;

Harbarth, S. ;

Buiting, A. G. M. ;

Crook, D. W. ;

Fitzpatrick, F. ;

Hanberger, H. ;

Herwaldt, L. A. ;

van Keulen, P. H. J. ;

Kluytmans, J. A. J. W. ;

Kola, A. ;

Kuchenbecker, R. S. ;

Lingaas, E. ;

Meessen, N. ;

Morris-Downes, M. M. ;

Pottinger, J. M. ;

Rohner, P. ;

dos Santos, R. P. ;

Seifert, H. ;

Wisplinghoff, H. ;

Ziesing, S. ;

Walker, A. S. ;

Bonten, M. J. M. .

CLINICAL INFECTIOUS DISEASES, 2013, 56 (06) :798-805

[2] Engineering an Endothelialized Vascular Graft: A Rational Approach to Study Design in a Non-Human Primate Model [J].

Anderson, Deirdre E. J. ;

Glynn, Jeremy J. ;

Song, Howard K. ;

Hinds, Monica T. .

PLOS ONE, 2014, 9 (12)

[3] Mixed bloodstream infections involving bacteria and Candida spp. [J].

Bouza, Emilio ;

Burillo, Almudena ;

Munoz, Patricia ;

Guinea, Jesus ;

Marin, Mercedes ;

Rodriguez-Creixems, Marta .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2013, 68 (08) :1881-1888

[4] Rapid Bacterial Identification, Resistance, Virulence and Type Profiling using Selected Reaction Monitoring Mass Spectrometry [J].

Charretier, Yannick ;

Dauwalder, Olivier ;

Franceschi, Christine ;

Degout-Charmette, Elodie ;

Zambardi, Gilles ;

Cecchini, Tiphaine ;

Bardet, Chloe ;

Lacoux, Xavier ;

Dufour, Philippe ;

Veron, Laurent ;

Rostaing, Herve ;

Lanet, Veronique ;

Fortin, Tanguy ;

Beaulieu, Corinne ;

Perrot, Nadine ;

Dechaume, Dominique ;

Pons, Sylvie ;

Girard, Victoria ;

Salvador, Arnaud ;

Durand, Geraldine ;

Mallard, Frederic ;

Theretz, Alain ;

Broyer, Patrick ;

Chatellier, Sonia ;

Gervasi, Gaspard ;

Van Nuenen, Marc ;

Roitsch, Carolyn Ann ;

Van Belkum, Alex ;

Lemoine, Jerome ;

Vandenesch, Francois ;

Charrier, Jean-Philippe .

SCIENTIFIC REPORTS, 2015, 5

[5] Principles of antibiotic therapy in severe infections: Optimizing the therapeutic approach by use of laboratory and clinical data [J].

Deresinski, Stan .

CLINICAL INFECTIOUS DISEASES, 2007, 45 :S177-S183

[6] Mass spectrometry-based proteomics combined with bioinformatic tools for bacterial classification [J].

Dworzanski, JP ;

Deshpande, SV ;

Chen, R ;

Jabbour, RE ;

Snyder, AP ;

Wick, CH ;

Li, L .

JOURNAL OF PROTEOME RESEARCH, 2006, 5 (01) :76-87

[7] Direct detection of extended-spectrum beta-lactamases (CTX-M) from blood cultures by LC-MS/MS bottom-up proteomics [J].

Fleurbaaij, F. ;

Goessens, W. ;

van Leeuwen, H. C. ;

Kraakman, M. E. M. ;

Bernards, S. T. ;

Hensbergen, P. J. ;

Kuijper, E. J. .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2017, 36 (09) :1621-1628

[8] Detection of amino acid substitutions in the GyrA protein of fluoroquinolone-resistant typhoidal Salmonella isolates using high-resolution mass spectrometry [J].

Hassing, Robert-Jan ;

Goessens, Wil H. ;

Zeneyedpour, Lona ;

Sultan, Sadaf ;

van Kampen, Jeroen J. ;

Verbon, Annelies ;

van Genderen, Perry J. ;

Hays, John P. ;

Luider, Theo M. ;

Dekker, Lennard J. .

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2016, 47 (05) :351-356

[9] Double-Blind Characterization of Non-Genome-Sequenced Bacteria by Mass Spectrometry-Based Proteomics [J].

Jabbour, Rabih E. ;

Deshpande, Samir V. ;

Wade, Mary Margaret ;

Stanford, Michael F. ;

Wick, Charles H. ;

Zulich, Alan W. ;

Skowronski, Evan W. ;

Snyder, A. Peter .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2010, 76 (11) :3637-3644

[10] Direct Identification and Antimicrobial Susceptibility Testing of Bacteria From Positive Blood Culture Bottles by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry and the Vitek 2 System [J].

Jo, Sung Jin ;

Park, Kang Gyun ;

Han, Kyungja ;

Park, Dong Jin ;

Park, Yeon-Joon .

ANNALS OF LABORATORY MEDICINE, 2016, 36 (02) :117-123

← 1 2 3 4 →