Quantitative detection of C-deuterated drugs by CARS microscopy and Raman microspectroscopy

被引：45

作者：

Bergner, Gero ^{[2
]}

Albert, Christian R. ^{[3
]}

Schiller, Markus ^{[4
]}

Bringmann, Gerhard ^{[3
]}

Schirmeister, Tanja ^{[4
]}

Dietzek, Benjamin ^{[2
,5
]}

Niebling, Stephan ^{[1
]}

Schluecker, Sebastian ^{[1
]}

Popp, Juergen ^{[2
,5
]}

机构：

[1] Dept Phys, D-49069 Osnabruck, Germany

[2] Inst Photon Technol, D-07745 Jena, Germany

[3] Univ Wurzburg, Inst Organ Chem, D-97074 Wurzburg, Germany

[4] Univ Wurzburg, Inst Pharm & Food Chem, D-97074 Wurzburg, Germany

[5] Univ Jena, Inst Phys Chem, D-07743 Jena, Germany

来源：

ANALYST | 2011年 / 136卷 / 18期

关键词：

SCATTERING MICROSCOPY; COHERENT; ALKALOIDS; CELLS;

D O I：

10.1039/c0an00956c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The introduction of carbon-deuterium (C-D) bonds into drug compounds by organic synthesis is a non-invasive labelling approach, which does not alter the chemical and physiological properties of the drug itself. C-deuterated drugs exhibit characteristic vibrational signatures in the C-D stretching region around 2100-2300 cm(-1), which avoids spectral interference with contributions from a complex biological environment. In this paper, the quantitative detection of C-deuterated drugs by Raman microspectroscopy and single-band CARS microscopy is examined. Concentration-dependent studies on drugs with aliphatic and aromatic C-D moieties were performed in a two-channel microfluidic chip, using the corresponding non-deuterated (C-H) isotopologues as an internal reference.

引用

页码：3686 / 3693

页数：8

共 37 条

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