Fiber-optic fluorometer for microscale mapping of photosynthetic pigments in microbial communities

被引：17

作者：

Thar, R

Kühl, M

Holst, G

机构：

[1] Univ Copenhagen, Marine Biol Lab, DK-3000 Helsingor, Denmark

[2] Max Planck Inst Marine Microbiol, D-28359 Bremen, Germany

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2001年 / 67卷 / 06期

关键词：

D O I：

10.1128/AEM.67.6.2823-2828.2001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Microscale fluorescence measurements were performed in photosynthetic biofilms at a spatial resolution of 100 to 200 mum with a new fiber-optic fluorometer which allowed four different excitation and emission wavelengths and was configured for measuring phycobiliproteins, chlorophylls, and bacteriochlorophylls. We present details of the measuring system and describe examples of applications in different microbial communities.

引用

页码：2823 / 2828

页数：6

共 17 条

[1] MICROSCALE VERTICAL PROFILES OF N-2 FIXATION, PHOTOSYNTHESIS, O-2, CHLOROPHYLL-ALPHA, AND LIGHT IN A CYANOBACTERIAL ASSEMBLAGE [J].