Compact oleic acid in HAMLET

被引：32

作者：

Fast, J

Mossberg, AK

Nilsson, H

Svanborg, C

Akke, M

Linse, S

机构：

[1] Lund Univ, Dept Biophys Chem, SE-22100 Lund, Sweden

[2] Lund Univ, Dept Microbiol Immunol & Glycobiol, SE-22100 Lund, Sweden

来源：

FEBS LETTERS | 2005年 / 579卷 / 27期

关键词：

nuclear magnetic resonance; HAMLET; oleic acid; alpha-lactalbumin;

D O I：

10.1016/j.febslet.2005.08.089

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

HAMLET (human alpha-lactalbumin made lethal to tumor cells) is a complex between alpha-lactalbumin and oleic acid that induces apoptosis in tumor cells, but not in healthy cells. Heteronuclear nuclear magnetic resonance (NMR) spectroscopy was used to determine the structure of C-13-oleic acid in HAM-LET, and to study the N-15-labeled protein. Nuclear Overhauser enhancement spectroscopy shows that the two ends of the fatty acid are in close proximity and close to the double bond, indicating that the oleic acid is bound to HAMLET in a compact conformation. The data further show that HAMLET is a partly unfolded/molten globule-like complex under physiological conditions. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：6095 / 6100

页数：6

共 30 条

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