Combined transcriptome and metabolite profiling analyses provide insights into the chronic toxicity of carbaryl and acetamiprid to Apis mellifera larvae

被引：12

作者：

Gao, Jing ^{[1
]}

Yang, Yang ^{[1
]}

Ma, Shilong ^{[1
,2
]}

Liu, Feng ^{[3
]}

Wang, Qiang ^{[1
]}

Wang, Xing ^{[4
]}

Wu, Yanyan ^{[1
]}

Zhang, Li ^{[1
]}

Liu, Yongjun ^{[1
]}

Diao, Qingyun ^{[1
]}

Dai, Pingli ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Apicultural Res, Key Lab Pollinating Insect Biol Agr, Beijing 100093, Peoples R China

[2] Enshi Acad Agr Sci, Enshi 445002, Peoples R China

[3] Jiangxi Inst Apicultural Res, Nanchang 330201, Jiangxi, Peoples R China

[4] Beijing Apicultural Stn, Beijing 100029, Peoples R China

来源：

SCIENTIFIC REPORTS | 2022年 / 12卷 / 01期

基金：

北京市自然科学基金; 中国国家自然科学基金;

关键词：

HONEY-BEE; NEONICOTINOID INSECTICIDES; OXIDATIVE STRESS; CHRONIC EXPOSURE; REACTIVE OXYGEN; ANTIOXIDANT; PESTICIDES; BIOMARKERS; MECHANISM; ENZYMES;

D O I：

10.1038/s41598-022-21403-0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Despite many studies have revealed that developing honey bee (Apis mellifera) larvae are posting a high risk on exposure to insecticides, the toxicology information on bee larvae remain limited. The present study demonstrated the first assessment of the effects of no observed adverse effect concentration (NOAEC) of carbaryl (CR) and acetamiprid (ACE) on transcriptome and metabolome in honeybee larvae reared in vitro. Chronic exposure to carbaryl caused transcriptional disorders associated with oxidative stress. In addition, a series of metabolic homeostasis were disrupted by carbaryl stress, such amino acid metabolism, purine and pyrimidine metabolism and flavone and flavonol biosynthesis. The activities of enzymic biomarkers including GST, P450, CAT, AChE and SOD were not influenced by ACE stress, while the CR exposure slightly decreased the activity of CAT and SOD. Our results clearly show that ACE and CR display different potential to modulate transcriptome and metabolome associated with their different toxicity against bee larvae.

引用

页数：15

共 78 条

[1] Toxicity and biochemical changes in the honey bee Apis mellifera exposed to four insecticides under laboratory conditions [J].