Resazurin assay of radiation response in cultured cells

被引：128
作者：
Anoopkumar-Dukie, S
Carey, JB
Conere, T
O'Sullivan, E
van Pelt, FN
Allshire, A [1 ]
机构：
[1] Univ Coll Cork, Dept Pharmacol & Therapeut, Cork, Ireland
[2] Cork Univ Hosp, Dept Med Phys, Cork, Ireland
来源：
BRITISH JOURNAL OF RADIOLOGY | 2005年 / 78卷 / 934期
关键词：
D O I：
10.1259/bjr/54004230
中图分类号：
R8 [特种医学]; R445 [影像诊断学];
学科分类号：
1002 ; 100207 ; 1009 ;
摘要：
We describe use of resazurin reduction for measurement of cell response to irradiation as a simple and non-destructive assay that complements the conventional colony forming assay and can readily be applied to both adherent and non-adherent cell cultures. The resazurin method yields data comparable with the colony forming assay as well as to assay of DNA synthesis (BrdU incorporation), giving an OER (oxygen enhancement ratio) of 2.5 at 60% isoeffect level versus 3.1 for the colony forming assay. Intraday and interday precisions for the resazurin assay were 4.1% and 5.2%, respectively.
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页码：945 / 947
页数：3
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