Transformed but not normal hepatocytes express UCP2

被引：45

作者：

Carretero, MV ^{[1
]}

Torres, L ^{[1
]}

Latasa, U ^{[1
]}

García-Trevijano, ER ^{[1
]}

Prieto, J ^{[1
]}

Mato, JM ^{[1
]}

Avila, MA ^{[1
]}

机构：

[1] Univ Navarra, Fac Med, Dept Med Interna, Div Hepatol & Terapia Gen, E-31080 Pamplona, Spain

来源：

FEBS LETTERS | 1998年 / 439卷 / 1-2期

关键词：

uncoupling protein; hepatoma; mitochondrion; tumor marker; hypomethylation;

D O I：

10.1016/S0014-5793(98)01335-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Uncoupling protein 2 (UCP2) expression in liver is restricted to non-parenchymal cells. By means of differential display screening between normal rat liver and H4IIE hepatoma cells we have isolated a cDNA clone encompassing part of UCP2 cDNA. Northern blot analysis revealed that UCP2 is expressed in some hepatocarcinoma cell lines, while it is absent in adult hepatocytes. UCP2, mRNA in H4IIE cells was downregulated when cells were cultured for 36 h in 0.1% serum and its expression was restored upon addition of 10% serum or phorbol esters. Hypomethylation of UCP2 was observed in transformed UCP2 expressing cells. Our results indicate that UCP2 is expressed in some hepatocarcinoma cell lines and that serum components may participate in maintaining elevated UCP2 levels. (C) 1998 Federation of European Biochemical Societies.

引用

页码：55 / 58

页数：4

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