Separation of amino acid enantiomers VIA chiral derivatization and non-chiral gas chromatography

被引：39

作者：

Bertrand, Marylene ^{[1
,2
]}

Chabin, Annie ^{[1
,2
]}

Brack, Andre ^{[1
,2
]}

Westall, Frances ^{[1
,2
]}

机构：

[1] CNRS, Ctr Biophys Mol, F-45071 Orleans 2, France

[2] Univ Orleans, F-45067 Orleans 2, France

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1180卷 / 1-2期

关键词：

non-chiral capillary GC; derivatization; ethyl chloroformate; 2-chloropropanol; amino acid diastereomers;

D O I：

10.1016/j.chroma.2007.12.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two GC-MS methods for the enantioselective separation of the 20 proteinogenic amino acids are compared. Ethyl chloroformate and 2-chloropropanol were used to derivatize amino acid enantiomers. The diastereomers formed were separated on a non-chiral column by capillary gas chromatography. The separation performances were compared to those obtained when using non-chiral derivatization on a chiral column. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：131 / 137

页数：7

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