Holocentric Chromosomes of Luzula elegans Are Characterized by a Longitudinal Centromere Groove, Chromosome Bending, and a Terminal Nucleolus Organizer Region

被引：50

作者：

Heckmann, S. ^{[1
]}

Schroeder-Reiter, E. ^{[2
]}

Kumke, K. ^{[1
]}

Ma, L. ^{[1
]}

Nagaki, K. ^{[3
]}

Murata, M. ^{[3
]}

Wanner, G. ^{[2
]}

Houben, A. ^{[1
]}

机构：

[1] Leibniz Inst Plant Genet & Crop Plant Res IPK, DE-06466 Gatersleben, Germany

[2] Univ Munich, Dept Biol 1, Bioctr, Planegg Martinsried, Germany

[3] Okayama Univ, Inst Plant Sci & Resources, Kurashiki, Okayama, Japan

来源：

CYTOGENETIC AND GENOME RESEARCH | 2011年 / 134卷 / 03期

关键词：

CENH3; Centromere; Holocentric chromosome; Luzula elegans; Mitosis; rDNA; CENP-A; RDNA SITES; HISTONE H3; KARYOTYPE EVOLUTION; PLANT CHROMOSOMES; KINETOCHORE; LOCALIZATION; SEGREGATION; BEHAVIOR; NUCLEOSOMES;

D O I：

10.1159/000327713

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The structure of holocentric chromosomes was analyzed in mitotic cells of Luzula elegans. Light and scanning electron microscopy observations provided evidence for the existence of a longitudinal groove along each sister chromatid. The centromere-specific histone H3 variant, CENH3, colocalized with this groove and with microtubule attachment sites. The terminal chromosomal regions were CENH3-negative. During metaphase to anaphase transition, L. elegans chromosomes typically curved to a sickle-like shape, a process that is likely to be influenced by the pulling forces of microtubules along the holocentric axis towards the corresponding microtubule organizing regions. A single pair of 45S rDNA sites, situated distal to Arabidopsis-telomere repeats, was observed at the terminal region of one chromosome pair. We suggest that the 45S rDNA position in distal centromere-free regions could be required to ensure chromosome stability. Copyright (C) 2011 S. Karger AG, Basel

引用

页码：220 / 228

页数：9

共 48 条

[1] LOCALIZATION OF THE RIBOSOMAL GENES IN CAENORHABDITIS-ELEGANS CHROMOSOMES BY INSITU HYBRIDIZATION USING BIOTIN-LABELED PROBES
ALBERTSON, DG
[J]. EMBO JOURNAL, 1984, 3 (06) : 1227 - 1234
[2] THE KINETOCHORES OF CAENORHABDITIS-ELEGANS
ALBERTSON, DG
THOMSON, JN
[J]. CHROMOSOMA, 1982, 86 (03) : 409 - 428
[3] Epigenetic regulation of centromeric chromatin: old dogs, new tricks?
Allshire, Robin C.
Karpen, Gary H.
[J]. NATURE REVIEWS GENETICS, 2008, 9 (12) : 923 - 937
[4] Chromosome analysis and molecular characterization of highly repeated DNAs in the aphid Acyrthosiphon pisum (Aphididae, Hemiptera)
Bizzaro, D
Mandrioli, M
Zanotti, M
Giusti, M
Manicardi, GC
[J]. GENETICA, 2000, 108 (02) : 197 - 202
[5] Structural determinants for generating centromeric chromatin
Black, BE
Foltz, DR
Chakravarthy, S
Luger, K
Woods, VL
Cleveland, DW
[J]. NATURE, 2004, 430 (6999) : 578 - 582
[6] An epigenetic mark generated by the incorporation of CENP-A into centromeric nucleosomes
Black, Ben E.
Brock, Melissa A.
Bedard, Sabrina
Woods, Virgil L., Jr.
Cleveland, Don W.
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (12) : 5008 - 5013
[7] Centromere identity maintained by nucleosomes assembled with histone H3 containing the CENP-A targeting domain
Black, Ben E.
Jansen, Lars E. T.
Maddox, Paul S.
Foltz, Daniel R.
Desai, Arshad B.
Shah, Jagesh V.
Cleveland, Don W.
[J]. MOLECULAR CELL, 2007, 25 (02) : 309 - 322
[8] ULTRASTRUCTURE OF NON-LOCALIZED KINETOCHORES OF LUZULA AND CYPERUS
BRASELTON, JP
[J]. CHROMOSOMA, 1971, 36 (01) : 89 - +
[9] Cell division -: A histone-H3-like protein in C-elegans
Buchwitz, BJ
Ahmad, K
Moore, LL
Roth, MB
Henikoff, S
[J]. NATURE, 1999, 401 (6753) : 547 - 548
[10] Centromere assembly requires the direct recognition of CENP-A nucleosomes by CENP-N
Carroll, Christopher W.
Silva, Mariana C. C.
Godek, Kristina M.
Jansen, Lars E. T.
Straight, Aaron F.
[J]. NATURE CELL BIOLOGY, 2009, 11 (07) : 896 - U297

← 1 2 3 4 5 →