Holocentric Chromosomes of Luzula elegans Are Characterized by a Longitudinal Centromere Groove, Chromosome Bending, and a Terminal Nucleolus Organizer Region

被引：50

作者：

Heckmann, S. ^{[1
]}

Schroeder-Reiter, E. ^{[2
]}

Kumke, K. ^{[1
]}

Ma, L. ^{[1
]}

Nagaki, K. ^{[3
]}

Murata, M. ^{[3
]}

Wanner, G. ^{[2
]}

Houben, A. ^{[1
]}

机构：

[1] Leibniz Inst Plant Genet & Crop Plant Res IPK, DE-06466 Gatersleben, Germany

[2] Univ Munich, Dept Biol 1, Bioctr, Planegg Martinsried, Germany

[3] Okayama Univ, Inst Plant Sci & Resources, Kurashiki, Okayama, Japan

来源：

CYTOGENETIC AND GENOME RESEARCH | 2011年 / 134卷 / 03期

关键词：

CENH3; Centromere; Holocentric chromosome; Luzula elegans; Mitosis; rDNA; CENP-A; RDNA SITES; HISTONE H3; KARYOTYPE EVOLUTION; PLANT CHROMOSOMES; KINETOCHORE; LOCALIZATION; SEGREGATION; BEHAVIOR; NUCLEOSOMES;

D O I：

10.1159/000327713

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The structure of holocentric chromosomes was analyzed in mitotic cells of Luzula elegans. Light and scanning electron microscopy observations provided evidence for the existence of a longitudinal groove along each sister chromatid. The centromere-specific histone H3 variant, CENH3, colocalized with this groove and with microtubule attachment sites. The terminal chromosomal regions were CENH3-negative. During metaphase to anaphase transition, L. elegans chromosomes typically curved to a sickle-like shape, a process that is likely to be influenced by the pulling forces of microtubules along the holocentric axis towards the corresponding microtubule organizing regions. A single pair of 45S rDNA sites, situated distal to Arabidopsis-telomere repeats, was observed at the terminal region of one chromosome pair. We suggest that the 45S rDNA position in distal centromere-free regions could be required to ensure chromosome stability. Copyright (C) 2011 S. Karger AG, Basel

引用

页码：220 / 228

页数：9

共 48 条

[1] LOCALIZATION OF THE RIBOSOMAL GENES IN CAENORHABDITIS-ELEGANS CHROMOSOMES BY INSITU HYBRIDIZATION USING BIOTIN-LABELED PROBES [J].