Clinical and environmental isolates of Burkholderia pseudomallei from Brazil: Genotyping and detection of virulence gene

被引:5
作者
Pinheiro Gomes Bandeira, Tereza de Jesus [1 ,2 ]
Collares Maia Castelo-Branco, Debora de Souza [1 ]
Gadelha Rocha, Marcos Fabio [1 ,3 ]
Cordeiro, Rossana de Aguiar [1 ]
Ocadaque, Crister Jose [1 ]
Neto Paiva, Manoel de Arajo [1 ,4 ]
Nogueira Brilhante, Raimunda Samia [1 ]
Costa Sidrim, Jose Julio [1 ]
机构
[1] Univ Fed Ceara, Postgrad Program Med Microbiol, Specialized Med Mycol Ctr, Fortaleza, Ceara, Brazil
[2] Christus Sch Med, Fortaleza, Ceara, Brazil
[3] Univ Estadual Ceara, Postgrad Program Vet Sci, Fortaleza, Ceara, Brazil
[4] Fed Inst Educ Sci & Technol Ceara, Acarau, Ceara, Brazil
关键词
Burkholderia pseudomallei; Melioidosis; RAPD; TTSS gene; MELIOIDOSIS;
D O I
10.1016/j.apjtm.2017.09.004
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Objective: To evaluate the genetic diversity of clinical and environmental isolates of Burkholderia pseudomallei (B. pseudomallei) recovered in Ceara, Brazil, and screen these isolates for the presence of type three secretion system virulence gene. Methods: Nineteen B. pseudomallei isolates (9 from clinical cases and 10 from soils) were analyzed. Random amplified polymorphic DNA was performed with primers OPQ-2, OPQ-4 and OPQ-16 to evaluate the genetic diversity, and type three secretion system gene was detected through polymerase chain reaction. Results: Random amplified polymorphic DNA showed a genetic relatedness of approximately 50% among the tested B. pseudomallei isolates, which were grouped into two clades, of which the biggest ones comprised 18/19 isolates for primer OPQ-2, and 17/19 isolates for primer OPQ-16. Primer OPQ-4 grouped the isolates into three clades comprising 1/19, 3/19 and 15/19 isolates. Additionally, type three secretion system gene was detected in all tested isolates. Conclusions: This is an effort to type B. pseudomallei strains from Ceara, which is important for better understanding this pathogen, contributing for the epidemiological surveillance of melioidosis in this endemic region.
引用
收藏
页码:945 / 951
页数:7
相关论文
共 50 条
  • [31] Antibiotic Susceptibility of Clinical Burkholderia pseudomallei Isolates in Northeast Thailand from 2015 to 2018 and the Genomic Characterization of β-Lactam-Resistant Isolates
    Hii, Shirley Yi Fen
    Tandhavanant, Sarunporn
    Phunpang, Rungnapa
    Ekchariyawat, Peeraya
    Saiprom, Natnaree
    Chewapreecha, Claire
    Seng, Rathanin
    Thiansukhon, Ekkachai
    Morakot, Chumpol
    Sangsa, Narongchai
    Chayangsu, Sunee
    Chuananont, Somchai
    Tanwisaid, Kittisak
    Silakun, Wirayut
    Buasi, Noppol
    Chaisuksant, Seksan
    Hompleum, Tanin
    Chetchotisakd, Ploenchan
    Day, Nicholas P. J.
    Chantratita, Wasun
    Lertmemongkolchai, Ganjana
    West, T. Eoin
    Chantratita, Narisara
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2021, 65 (05)
  • [32] Molecular Investigation of Virulence Determinants between a Virulent Clinical Strain and an Attenuated Strain of Burkholderia pseudomallei
    Puthucheary, S. D.
    Puah, Suat Moi
    Chai, Hwa Chia
    Thong, Kwai Lin
    Chua, Kek Heng
    JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY, 2012, 22 (03) : 198 - 204
  • [33] The BpeEF-OprC Efflux Pump Is Responsible for Widespread Trimethoprim Resistance in Clinical and Environmental Burkholderia pseudomallei Isolates
    Podnecky, Nicole L.
    Wuthiekanun, Vanaporn
    Peacock, Sharon J.
    Schweizer, Herbert P.
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2013, 57 (09) : 4381 - 4386
  • [34] The effect of methanolic extract of Tamarindus indica Linn. on the growth of clinical isolates of Burkholderia pseudomallei
    Muthu, SE
    Nandakumar, S
    Rao, UA
    INDIAN JOURNAL OF MEDICAL RESEARCH, 2005, 122 (06) : 525 - 528
  • [35] Phenotype and Virulence Assessment of a Burkholderia pseudomallei Soil Isolate from Malaysia
    Wong, Rui-Rui
    Baharom, Khairom-Muslihin
    Ghazali, Ahmad-Kamal
    Hassan, Abdul Karim Russ
    Nathan, Sheila
    SAINS MALAYSIANA, 2021, 50 (05): : 1233 - 1241
  • [36] Ribotyping and DNA macrorestriction analysis of isolates of Burkholderia pseudomallei from cases of melioidosis in Malaysia
    Vadivelu, J
    Puthucheary, SD
    Mifsud, A
    Drasar, BS
    Dance, DAB
    Pitt, TL
    TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1997, 91 (03) : 358 - 360
  • [37] Burkholderia pseudomallei animal and human isolates from Malaysia exhibit different phenotypic characteristics
    Lee, Song-Hua
    Chong, Chan-Eng
    Lim, Boon-San
    Chai, San-Jiun
    Sam, Kin-Kit
    Mohamed, Rahmah
    Nathan, Sheila
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2007, 58 (03) : 263 - 270
  • [38] Characterization and mutagenesis of fur gene from Burkholderia pseudomallei
    Loprasert, S
    Sallabhan, R
    Whangsuk, W
    Mongkolsuk, S
    GENE, 2000, 254 (1-2) : 129 - 137
  • [39] Antimicrobial susceptibility pattern of clinical isolates of Burkholderia pseudomallei in Bangladesh
    Dutta S.
    Haq S.
    Hasan M.R.
    Haq J.A.
    BMC Research Notes, 10 (1)
  • [40] Development and validation of TagMan real-time PCR for the detection of Burkholderia pseudomallei isolates from Malaysia
    Ali, Mohd M. R.
    Foo, P. C.
    Hassan, M.
    Maning, N.
    Hussin, A.
    Yunus, Syed Ahmad S. Z.
    Fauzi, M. H.
    Besar, Muhd A.
    Harlin, A.
    Ismail, N.
    Chan, Y. Y.
    TROPICAL BIOMEDICINE, 2019, 36 (02) : 379 - 389