Prevalence and Genetic Characterization of Toxoplasma gondii in Pet Dogs in Central China

被引:15
|
作者
Qian, Wei-Feng [1 ]
Yan, Wen-Chao [1 ]
Wang, Tian-Qi [1 ]
Zhai, Kai [1 ]
Han, Li-Fang [1 ]
Lv, Chao-Chao [2 ]
机构
[1] Henan Univ Sci & Technol, Coll Anim Sci & Technol, Luoyang 471003, Peoples R China
[2] PuLike Biol Engn Co Ltd, Luoyang 471000, Peoples R China
关键词
Toxoplasma gondii; prevalence; genotype; dogs; China; NORTHEASTERN CHINA; 1ST REPORT; INFECTION; SEROPREVALENCE; GENOTYPE; PROVINCE; LANZHOU; BIRDS; STRAY; CATS;
D O I
10.3347/kjp.2015.53.1.125
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The prevalence and genotype of Toxoplasma gondii infection in dogs in Henan Province, Central China was investigated. A total of 125 blood samples were collected from pet dogs during April to June 2013, and all samples were examined by indirect hemagglutination antibody test (IHA) and nested PCR. The overall T. gondii prevalence in pet dogs was 24.0% (30/125), with 20.8% (26/125) in IHA and 10.4% (13/125) in PCR, respectively. No statistical associations were found between animal gender and age and the prevalence of T. gondii infection. Thirteen positive DNA samples were genotyped using 11 PCR-RFLP markers, including SAG1, (3'+5') SAG2, alt. SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico. Of these, only 2 samples were genotyped with complete data for all loci, and a novel genotype (type III at SAG3 and GRA6 loci, and type I at other loci) was identified. This is the first report of genetic characterization of T. gondii infection in dogs in China.
引用
收藏
页码:125 / 128
页数:4
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