Molecular detection of Toxoplasma gondii in chicken hearts from markets and retail stores in Northern Iran

被引：6

作者：

Abbaszadeh, Somayeh ^{[1
]}

Teimouri, Aref ^{[2
]}

Mahmoudi, Mohammad Reza ^{[1
]}

Roushan, Zahra Atrkar ^{[3
]}

Hajipour, Nayereh ^{[4
]}

Majidi-Shad, Bijan ^{[1
]}

Sharifdini, Meysam ^{[1
]}

机构：

[1] Guilan Univ Med Sci, Sch Med, Dept Med Parasitol & Mycol, Rasht, Iran

[2] Shiraz Univ Med Sci, Sch Med, Dept Parasitol & Mycol, Shiraz, Iran

[3] Guilan Univ Med Sci, Sch Med, Dept Biostat, Rasht, Iran

[4] Guilan Univ Med Sci, Sch Med, Dept Microbiol, Rasht, Iran

来源：

FOOD AND WATERBORNE PARASITOLOGY | 2022年 / 27卷

关键词：

Toxoplasma gondii; Chickens; Northern Iran; B1; gene; SAG1; GALLUS-DOMESTICUS; TISSUE CYSTS; INFECTION; SENTINEL; STRAINS;

D O I：

10.1016/j.fawpar.2022.e00166

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Detection of Toxoplasma gondii in chicken products indicates risk of transmission to consumers. The objective of the current study was to investigate the molecular prevalence of T. gondii in freeranging and industrial chickens in Guilan province, Northern Iran. A total of 150 chicken heart samples including 75 free-range and 75 industrial chickens were collected from farmers' markets and chicken retailers in Guilan, Northern Iran, between October 2017 and August 2018. Genomic DNA were extracted from samples and examined for evidence of T. gondii using polymerase chain reaction (PCR) targeting the B1 gene. The B1-positive samples were further analyzed by nestedPCR for SAG1 gene. Of the 150 samples, T. gondii DNA fragments were detected in 59 (39.3%), including 30 (40%) free-range and 29 (38.7%) industrial chicken. No significant differences of T. gondii DNA detection was observed between the free-range and industrial chicken samples (p = 0.73). Four selected positive samples were used for amplifying and sequencing of the SAG1 gene. The results revealed that all four sequences of SAG1 had 100% similarity with T. gondii sequences previously isolated from an AIDS/HIV patient in Mazandaran province, Northern Iran. Furthermore, the phylogenetic analysis demonstrated that all four sequences were closely related to Type I of T. gondii. However, our Type I identification is preliminary and needs to be confirmed by further multilocus sequence typing (MLST) analysis. The findings of the present study provide new data about the presence of T. gondii DNA in chicken hearts in the study area. These results confirm that chicken can be used as sentinels for environment contamination; however, further studies are needed to determine the viability of T. gondii in chicken hearts from Iran for risk assessment.

引用

页数：6

共 36 条

[1]

Asgari Q., 2008, INT J POULT SCI, V7, P1247

[2] Sources of toxoplasma infection in pregnant women: European multicentre case-control study [J].

Cook, AJC ;

Gilbert, RE ;

Buffolano, W ;

Zufferey, J ;

Petersen, E ;

Jenum, PA ;

Foulon, W ;

Semprini, AE ;

Dunn, DT .

BRITISH MEDICAL JOURNAL, 2000, 321 (7254) :142-147

[3] Epidemiologic significance ofToxoplasma gondiiinfections in chickens (Gallus domesticus): the past decade [J].

Dubey, J. P. ;

Pena, H. F. J. ;

Cerqueira-Cezar, C. K. ;

Murata, F. H. A. ;

Kwok, O. C. H. ;

Yang, Y. R. ;

Gennari, S. M. ;

Su, C. .

PARASITOLOGY, 2020, 147 (12) :1263-1289

[4] Toxoplasmosis in sentinel chickens (Gallus domesticus) in New England farms: Seroconversion, distribution of tissue cysts in brain, heart, and skeletal muscle by bioassay in mice and cats [J].

Dubey, J. P. ;

Lehmann, T. ;

Lautner, F. ;

Kwok, O. C. H. ;

Gamble, H. R. .

VETERINARY PARASITOLOGY, 2015, 214 (1-2) :55-58

[5] Toxoplasma gondii Infections in Chickens (Gallus domesticus): Prevalence, Clinical Disease, Diagnosis and Public Health Significance [J].

Dubey, J. P. .

ZOONOSES AND PUBLIC HEALTH, 2010, 57 (01) :60-73

[6] Toxoplasma gondii isolates from free-ranging chickens from the United States [J].

Dubey, JP ;

Graham, DH ;

Dahl, E ;

Sreekumar, C ;

Lehmann, T ;

Davis, MF ;

Morishita, TY .

JOURNAL OF PARASITOLOGY, 2003, 89 (05) :1060-1062

[7] Isolation and molecular characterization of Toxoplasma gondii from chickens and ducks from Egypt [J].

Dubey, JP ;

Graham, DH ;

Dahl, E ;

Hilali, M ;

El-Ghaysh, A ;

Sreekumar, C ;

Kwok, OCH ;

Shen, SK ;

Lehmann, T .

VETERINARY PARASITOLOGY, 2003, 114 (02) :89-95

[8] Biological and genetic characterisation of Toxoplasma gondii isolates from chickens (Gallus domesticus) from Sao Paulo, Brazil:: unexpected findings [J].

Dubey, JP ;

Graham, DH ;

Blackston, CR ;

Lehmann, T ;

Gennari, SM ;

Ragozo, AMA ;

Nishi, SM ;

Shen, SK ;

Kwok, OCH ;

Hilla, DE ;

Thulliez, P .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2002, 32 (01) :99-105

[9]

Dubey JP, 2010, Toxoplasmosis of animals and humans, V2nd, DOI DOI 10.1201/9781003199373

[10]

Eslami A., 2007, Iranian Journal of Parasitology, V2, P42

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