Human endothelial progenitor cells-derived exosomes accelerate cutaneous wound healing in diabetic rats by promoting endothelial function

被引：145
作者：
Li, Xiaocong [1 ]
Jiang, Chunyu [1 ]
Zhao, Jungong [1 ]
机构：
[1] Shanghai Jiao Tong Univ, Affiliated Peoples Hosp 6, Dept Radiol, 600 Yishan Rd, Shanghai 200233, Peoples R China
来源：
JOURNAL OF DIABETES AND ITS COMPLICATIONS | 2016年 / 30卷 / 06期
基金：
中国国家自然科学基金;
关键词：
Endothelial progenitor cells; Exosomes; Diabetes; Angiogenesis; Wound healing; STEM-CELLS; VASCULAR INJURY; MICROVESICLES; TRANSPLANTATION; ANGIOGENESIS; ISCHEMIA; REPAIR;
D O I：
10.1016/j.jdiacomp.2016.05.009
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Aims: Wound healing is deeply dependent on neovascularization to restore blood flow. The neovascularization of endothelial progenitor cells (EPCs) through paracrine secretion has been reported in various tissue repair models. Exosomes, key components of cell paracrine mechanism, have been rarely reported in wound healing. Methods: Exosomes were isolated from the media of EPCs obtained from human umbilical cord blood. Diabetic rats wound model was established and treated with exosomes. The in vitro effects of exosomes on the proliferation, migration and angiogenic tubule formation of endothelial cells were investigated. Results: We revealed that human umbilical cord blood EPCs derived exosomes transplantation could accelerate cutaneous wound healing in diabetic rats. We also showed that exosomes enhanced the proliferation, migration and tube formation of vascular endothelial cells in vitro. Furthermore, we found that endothelial cells stimulated with these exosomes would increase expression of angiogenesis-related molecules, including FGF-1, VEGFA, VEGFR-2, ANG-1, E-selectin, CXCL-16, eNOS and IL-8. Conclusion: Taken together, our findings indicated that EPCs-derived exosomes facilitate wound healing by positively modulating vascular endothelial cells function. (C) 2016 Elsevier Inc. All rights reserved.
引用
收藏
页码：986 / 992
页数：7
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