Light-Induced Protein Dimerization by One- and Two-Photon Activation of Gibberellic Acid Derivatives in Living Cells

被引：52

作者：

Schelkle, Korwin M. ^{[2
,3
]}

Griesbaum, Tristan ^{[1
]}

Ollech, Dirk ^{[1
]}

Becht, Steffy ^{[4
]}

Buckup, Tiago ^{[4
]}

Hamburger, Manuel ^{[2
,3
]}

Wombacher, Richard ^{[1
]}

机构：

[1] Heidelberg Univ, Inst Pharm & Mol Biotechnol, D-69120 Heidelberg, Germany

[2] Heidelberg Univ, Inst Organ Chem, D-69120 Heidelberg, Germany

[3] InnovationLab GmbH, D-69115 Heidelberg, Germany

[4] Heidelberg Univ, Inst Phys Chem, D-69120 Heidelberg, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2015年 / 54卷 / 09期

关键词：

caged compounds; chemical inducer of dimerization; photolysis; protein-protein interactions; two-photon activation; PHOTOACTIVATED GENE-EXPRESSION; MAMMALIAN-CELLS; RECEPTOR; SYSTEM; PHOTOCONTROL; GLUTAMATE; RELEASE; ANALOG; GID1; NM;

D O I：

10.1002/anie.201409196

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We developed a highly efficient system for light-induced protein dimerization in live cells using photo-caged derivatives of the phytohormone gibberellic acid (GA(3)). We demonstrate the application of the photo-activatable chemical inducer of dimerization (CID) for the control of protein translocation with high spatiotemporal precision using light as an external trigger. Furthermore, we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA(3) for inducing protein dimerization in living cells.

引用

页码：2825 / 2829

页数：5

共 42 条

[1] CONTROLLING CELL CHEMISTRY WITH CAGED COMPOUNDS [J].

ADAMS, SR ;

TSIEN, RY .

ANNUAL REVIEW OF PHYSIOLOGY, 1993, 55 :755-784

[2] A versatile synthetic dimerizer for the regulation of protein-protein interactions [J].

Amara, JF ;

Clackson, T ;

Rivera, VM ;

Guo, T ;

Keenan, T ;

Natesan, S ;

Pollock, R ;

Yang, W ;

Courage, NL ;

Holt, DA ;

Gilman, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (20) :10618-10623

[3]

[Anonymous], 2006, ANGEW CHEM, V118, P2287

[4]

Brieke C., 2012, ANGEW CHEM, V124, P8572

[5] Light-Controlled Tools [J].

Brieke, Clara ;

Rohrbach, Falk ;

Gottschalk, Alexander ;

Mayer, Guenter ;

Heckel, Alexander .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2012, 51 (34) :8446-8476

[6] A caged doxycycline analogue for photoactivated gene expression [J].

Cambridge, SB ;

Geissler, D ;

Keller, S ;

Cürten, B .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (14) :2229-2231

[7] Doxycycline-dependent photoactivated gene expression in eukaryotic systems [J].

Cambridge, Sidney B. ;

Geissler, Daniel ;

Calegari, Federico ;

Anastassiadis, Konstantinos ;

Hasan, Mazahir T. ;

Stewart, A. Francis ;

Huttner, Wieland B. ;

Hagen, Volker ;

Bonhoeffer, Tobias .

NATURE METHODS, 2009, 6 (07) :527-U86

[8] Manipulating signaling at will: chemically-inducible dimerization (CID) techniques resolve problems in cell biology [J].

DeRose, Robert ;

Miyamoto, Takafumi ;

Inoue, Takanari .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2013, 465 (03) :409-417

[9]

Donato L., 2012, Angew. Chem, V124, P1876

[10] Water-Soluble, Donor-Acceptor Biphenyl Derivatives in the 2-(o-Nitrophenyl)propyl Series: Highly Efficient Two-Photon Uncaging of the Neurotransmitter ?-Aminobutyric Acid at ?=800 nm [J].

Donato, Loic ;

Mourot, Alexandre ;

Davenport, Christopher M. ;

Herbivo, Cyril ;

Warther, David ;

Leonard, Jeremie ;

Bolze, Frederic ;

Nicoud, Jean-Francois ;

Kramer, Richard H. ;

Goeldner, Maurice ;

Specht, Alexandre .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2012, 51 (08) :1840-1843

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