Control of gene editing by manipulation of DNA repair mechanisms

被引：51

作者：

Danner, Eric ^{[1
]}

Bashir, Sanum ^{[1
,2
]}

Yumlu, Saniye ^{[1
,2
]}

Wurst, Wolfgang ^{[3
,4
,5
,6
]}

Wefers, Benedikt ^{[3
,4
]}

Kuehn, Ralf ^{[1
,2
]}

机构：

[1] Max Delbruck Centrum Mol Med, Robert Rossle Str 10, D-13125 Berlin, Germany

[2] Berlin Inst Hlth, Kapelle Ufer 2, D-10117 Berlin, Germany

[3] Deutsch Zentrum Neurodegenerat Erkrankungen eV DZ, Feodor Lynen Str 17, D-81377 Munich, Germany

[4] Helmholtz Zentrum Munchen, Inst Dev Genet, German Res Ctr Environm Hlth, D-85764 Neuherberg, Germany

[5] Tech Univ Munchen Weihenstephan, Chair Dev Genet, Helmholtz Zentrum Munchen, Ingolstadter Landstr 1, D-85764 Neuherberg, Germany

[6] Munich Cluster Syst Neurol SyNergy, Feodor Lynen Str 17, D-81377 Munich, Germany

来源：

MAMMALIAN GENOME | 2017年 / 28卷 / 7-8期

关键词：

STRAND BREAK REPAIR; HOMOLOGY-DIRECTED REPAIR; KNOCK-IN; END RESECTION; MOUSE MODEL; GENOME; CRISPR-CAS9; RECOMBINATION; 53BP1; DISTINCT;

D O I：

10.1007/s00335-017-9688-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA double-strand breaks (DSBs) are produced intentionally by RNA-guided nucleases to achieve genome editing through DSB repair. These breaks are repaired by one of two main repair pathways, classic non-homologous end joining (c-NHEJ) and homology-directed repair (HDR), the latter being restricted to the S/G2 phases of the cell cycle and notably less frequent. Precise genome editing applications rely on HDR, with the abundant c-NHEJ formed mutations presenting a barrier to achieving high rates of precise sequence modifications. Here, we give an overview of HDR- and c-NHEJ-mediated DSB repair in gene editing and summarize the current efforts to promote HDR over c-NHEJ.

引用

页码：262 / 274

页数：13

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