Automating parallel peptide synthesis for the production of PNA library arrays

被引:24
作者
Matysiak, S [1 ]
Reuthner, F [1 ]
Hoheisel, JD [1 ]
机构
[1] Deutsch Krebsforschungszentrum, D-69120 Heidelberg, Germany
关键词
D O I
10.2144/01314rr01
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A system was established for the parallel synthesis of peptide library arrays in a fully automated manner Synthesis takes place in blocks made of polyoxymethylene that hold during all synthesis steps a polypropylene membrane of 8 x 12 cm. Yields are in the nanomole range, obtained at a low consumption of reagents. The current setup is based on a commercially available pipetting robot-and supports the generation of 1536 different oligomers/run. Much higher array densities are possible because the membranes are amicable to spot diameters of down to 200 mum, naturally at a cost of the absolute amount produced of each oligomer. The method was put to use for the creation of arrayed libraries of peptide nucleic acids (PNAs). These can be employed both as a source of PNA molecules applied individually in experimentation subsequent to their release or as intact oligomer arrays in hybridization analyses.
引用
收藏
页码:896 / +
页数:6
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