LncRNA-TP53TG1 Participated in the Stress Response Under Glucose Deprivation in Glioma

被引：53

作者：

Chen, Xin ^{[1
]}

Gao, Yang ^{[1
]}

Li, Deheng ^{[1
]}

Cao, Yiqun ^{[1
]}

Hao, Bin ^{[1
]}

机构：

[1] Fudan Univ, Dept Brain & Spine Surg, Shanghai Canc Ctr, 270 Dongan Rd, Shanghai 200032, Peoples R China

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2017年 / 118卷 / 12期

基金：

中国国家自然科学基金;

关键词：

GLIOMAS; TP53TG1; TUMOR GLUCOSE METABOLISM; PROLIFERATION; MIGRATION; PYRUVATE-KINASE; CANCER; PROLIFERATION; EXPRESSION; GENE; MICROENVIRONMENT; MUTATIONS; INDUCTION; MIGRATION; PROMOTES;

D O I：

10.1002/jcb.26175

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gliomas are the most common brain tumors of the center nervous system. And long non-coding RNAs (lncRNAs) are non-protein coding transcripts, which have been considered as one type of gene expression regulator for cancer development. In this study, we investigated the role of lncRNA-TP53TG1 in response to glucose deprivation in human gliomas. The expression levels of TP53TG1 in glioma tissues and cells were analyzed by qRT-PCR. In addition, the influence of TP53TG1 on glucose metabolism related genes at the mRNA level during both high and low glucose treatment was detected by qRT-PCR. MTT, clonogenicity assays, and flow cytometry were performed to detect the cell proliferation and cell apoptosis. Furthermore, the migration of glioma cells was examined by Transwell assays. The expression of TP53TG1 was significantly higher in human glioma tissues or cell lines compared with normal brain tissue or NHA. Moreover, TP53TG1 and some tumor glucose metabolism related genes, such as GRP78, LDHA, and IDH1 were up-regulated significantly in U87 and LN18 cells under glucose deprivation. In addition, knockdown of TP53TG1 decreased cell proliferation and migration and down-regulated GRP78 and IDH1 expression levels and up-regulated PKM2 levels in U87 cells under glucose deprivation. However, over-expression of TP53TG1 showed the opposite tendency. Moreover, the effects of TP53TG1 were more remarkable in low glucose than that in high glucose. Our data showed that TP53TG1 under glucose deprivation may promote cell proliferation and migration by influencing the expression of glucose metabolism related genes in glioma. J. Cell. Biochem. 118: 4897-4904, 2017. (c) 2017 Wiley Periodicals, Inc.

引用

页码：4897 / 4904

页数：8

共 36 条

[1] Inhibition of Pyruvate Kinase M2 by Reactive Oxygen Species Contributes to Cellular Antioxidant Responses [J].

Anastasiou, Dimitrios ;

Poulogiannis, George ;

Asara, John M. ;

Boxer, Matthew B. ;

Jiang, Jian-kang ;

Shen, Min ;

Bellinger, Gary ;

Sasaki, Atsuo T. ;

Locasale, Jason W. ;

Auld, Douglas S. ;

Thomas, Craig J. ;

Vander Heiden, Matthew G. ;

Cantley, Lewis C. .

SCIENCE, 2011, 334 (6060) :1278-1283

[2]

Cohen N, 2007, NEUROPATHOL APPL NEU, V33, P710, DOI DOI 10.1111/J.1365-2990.2007.00905.X

[3] The peroxisome proliferator-activated receptor-γ agonist troglitazone inhibits transforming growth factor-β-mediated glioma cell migration and brain invasion [J].

Coras, Roland ;

Hoelsken, Annett ;

Seufert, Sebastian ;

Hauke, Jan ;

Eyuepoglu, Ilker Y. ;

Reichel, Martin ;

Traenkle, Christian ;

Siebzehnruebl, Florian A. ;

Buslei, Rolf ;

Bluemcke, Ingmar ;

Hahnen, Eric .

MOLECULAR CANCER THERAPEUTICS, 2007, 6 (06) :1745-1754

[4] The prostate cancer-up-regulated long noncoding RNA PlncRNA-1 modulates apoptosis and proliferation through reciprocal regulation of androgen receptor [J].

Cui, Zilian ;

Ren, Shancheng ;

Lu, Ji ;

Wang, Fubo ;

Xu, Weidong ;

Sun, Yi ;

Wei, Min ;

Chen, Junyi ;

Gao, Xu ;

Xu, Chuanliang ;

Mao, Jian-Hua ;

Sun, Yinghao .

UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS, 2013, 31 (07) :1117-1123

[5] Oncogenic alterations of metabolism [J].

Dang, CV ;

Semenza, GL .

TRENDS IN BIOCHEMICAL SCIENCES, 1999, 24 (02) :68-72

[6] Epigenetic inactivation of the p53-induced long noncoding RNA TP53 target 1 in human cancer [J].

Diaz-Lagares, Angel ;

Crujeiras, Ana B. ;

Lopez-Serra, Paula ;

Soler, Marta ;

Setien, Fernando ;

Goyal, Ashish ;

Sandoval, Juan ;

Hashimoto, Yutaka ;

Martinez-Cardus, Anna ;

Gomez, Antonio ;

Heyn, Holger ;

Moutinho, Catia ;

Espada, Jesus ;

Vidal, August ;

Paules, Maria ;

Galan, Maica ;

Sala, Nuria ;

Akiyama, Yoshimitsu ;

Martinez-Iniesta, Maria ;

Farre, Lourdes ;

Villanueva, Alberto ;

Gross, Matthias ;

Diederichs, Sven ;

Guil, Sonia ;

Esteller, Manel .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (47) :E7535-E7544

[7] Expression of Far Upstream Element (FUSE) Binding Protein 1 in Human Glioma is Correlated With c-Myc and Cell Proliferation [J].

Ding, Zongmei ;

Liu, Xiancheng ;

Liu, Yonghua ;

Zhang, Jianguo ;

Huang, Xianting ;

Yang, Xiaojing ;

Yao, Li ;

Cui, Gang ;

Wang, Donglin .

MOLECULAR CARCINOGENESIS, 2015, 54 (05) :405-415

[8] Emerging insights into the molecular and cellular basis of glioblastoma [J].

Dunn, Gavin P. ;

Rinne, Mikael L. ;

Wykosky, Jill ;

Genovese, Giannicola ;

Quayle, Steven N. ;

Dunn, Ian F. ;

Agarwalla, Pankaj K. ;

Chheda, Milan G. ;

Campos, Benito ;

Wang, Alan ;

Brennan, Cameron ;

Ligon, Keith L. ;

Furnari, Frank ;

Cavenee, Webster K. ;

Depinho, Ronald A. ;

Chin, Lynda ;

Hahn, William C. .

GENES & DEVELOPMENT, 2012, 26 (08) :756-784

[9]

Gibb EA, 2010, PLOS ONE, V6

[10] Glucose deprivation activates a metabolic and signaling amplification loop leading to cell death [J].

Graham, Nicholas A. ;

Tahmasian, Martik ;

Kohli, Bitika ;

Komisopoulou, Evangelia ;

Zhu, Maggie ;

Vivanco, Igor ;

Teitell, Michael A. ;

Wu, Hong ;

Ribas, Antoni ;

Lo, Roger S. ;

Mellinghoff, Ingo K. ;

Mischel, Paul S. ;

Graeber, Thomas G. .

MOLECULAR SYSTEMS BIOLOGY, 2012, 8

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