Viability of Mesenchymal Stem Cells in an Ex Vivo Circulation System

被引：3

作者：

Cho, Hwa Jin ^{[1
,2
]}

Hong, Hyun ^{[2
,3
]}

Kim, Do Wan ^{[2
,3
]}

Lee, Kyo Seon ^{[2
,3
]}

Han, Hwa Seon ^{[1
,2
]}

Kim, Geum Hee ^{[2
,3
]}

Choi, Kyung Soon ^{[2
,3
]}

Kim, Yong Sook ^{[4
]}

Kayumov, Mukhammad ^{[2
,3
]}

Ki, Katrina K. ^{[5
,6
]}

Suen, Jacky ^{[5
,6
]}

Fraser, John ^{[5
,6
,7
]}

Jeong, In Seok ^{[2
,3
]}

机构：

[1] Chonnam Natl Univ, Dept Pediat, Childrens Hosp, Gwangju, South Korea

[2] Med Sch, Gwangju, South Korea

[3] Chonnam Natl Univ Hosp, Dept Thorac & Cardiovasc Surg, 42 Jebong Ro, Gwangju 501757, South Korea

[4] Chonnam Natl Univ Hosp, Biomed Res Inst, Gwangju, South Korea

[5] Univ Queensland, Fac Med, Crit Care Res Grp, Brisbane, Qld, Australia

[6] Prince Charles Hosp, Brisbane, Qld, Australia

[7] Prince Charles Hosp, Innovat Cardiovasc Engn & Technol Lab, Crit Care Res Grp, Brisbane, Qld, Australia

来源：

ASAIO JOURNAL | 2020年 / 66卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

extracorporeal membrane oxygenation (ECMO); mesenchymal stem cell; viability; activity; ex-vivo; EXTRACORPOREAL MEMBRANE-OXYGENATION; HEART-FAILURE; THERAPY; TRANSPLANTATION; PERFUSION; SUPPORT; INJURY; BRIDGE;

D O I：

10.1097/MAT.0000000000001025

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Extracorporeal membrane oxygenation (ECMO) is a well-known therapy for refractory cardiac and respiratory failure. Stem cell therapy has been investigated as an adjunctive treatment for use during ECMO, but little is known about the viability of stem cells during ECMO support. We evaluated the viability and activity of mesenchymal stem cells (MSCs) in ex vivo circulation (EVC) conditions. The experimental groups were divided into two subgroups: EVC with oxygenator (OXY group) and EVC without oxygenator (Non-OXY group). Mesenchymal stem cells (1.0 x 10(7)) were injected into the EVC system. Cell counting, a lactate dehydrogenase (LDH) cytotoxicity assay, and the mitochondrial functions of viable MSCs were analyzed. The post-EVC oxygen consumption rate (OCR) was significantly lower than the pre-EVC OCR, regardless of whether the oxygenator was used. The LDH levels were significantly higher in the OXY group than in the Non-OXY group. The cellular loss was mainly due to lysis of the cells whereas the loss of cellular activity was attributed to the nonphysiologic condition itself, as well as the oxygenator. We concluded that direct infusion of MSCs during ECMO support did not serve as adjunctive therapy. Further studies are needed to improve the viability in an ECMO setting.

引用

页码：433 / 440

页数：8

共 24 条

[11] The Use of GMP-Produced Bone Marrow-Derived Stem Cells in Combination with Extracorporeal Membrane Oxygenation in ARDS: An Animal Model [J].