Smart platform for the time since death determination from vitreous humor cystine

In this work, we report the smart application of AgNPs based sensors for determination of time since death (TSD) via recognition and quantification of vitreous humor (VH) cystine as well as provide the portability for on spot determination of TSD. The lower detection limit was found to be 7.0 ng/ml with prominent selectivity. It was found that there is a linear correlation between the VH cystine concentration and TSD as the concentration of cystine increases up to 96 h +/- 3.9 h. Further for the first time TSD determination is given a smart approach and it proves to have a great utility up to 24 h +/- 2.6 h. The linear regression equation between TSD (the dependent variable), RGB intensity of cystine concentration till 24 h (the independent variable) was found to be TSD=26.69+-0.05*x. The proposed method gives the smart detection, portability, rapidity, sensitivity, selectivity as well as cost effectiveness for determination of time since death. (C) 2016 Elsevier B.V. All rights reserved.