Glucose Biosensor Based on the Fabrication of Glucose Oxidase in the Bio-Inspired Polydopamine-Gold Nanoparticle Composite Film

被引：10

作者：

Wei, Yinyin ^{[1
]}

Zhang, Ningdan ^{[1
]}

Li, Ying ^{[1
]}

Shi, Guoyue ^{[1
]}

Jin, Litong ^{[1
]}

机构：

[1] E China Normal Univ, Dept Chem, Shanghai 200062, Peoples R China

来源：

CHINESE JOURNAL OF CHEMISTRY | 2010年 / 28卷 / 12期

基金：

中国国家自然科学基金;

关键词：

polydopamine films; gold nanoparticles; glucose oxidase; glucose biosensor; IMMOBILIZATION; CHEMISTRY; SENSOR; SIZE;

D O I：

10.1002/cjoc.201190027

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A highly efficient enzyme immobilization method has been developed for electrochemical biosensors using polydopamine films with gold nanoparticles (AuNPs) embedded. This simple enzyme fabrication method can be performed in very mild conditions and stored in a long time with high bioactivity. The fabricated amperometric glucose biosensor exhibited a high and reproducible sensitivity, wide linear dynamic range and low limit of detection (LOD) (0.1 mu mol.L-1). A low value of 1.5 mmol.L-1 for the apparent Michaelis-Menten constant K-M(app) was obtained. The high sensitivity, wide linear range, good reproducibility and stability make this biosensor a promising candidate for portable amperometric glucose biosensor.

引用

页码：2489 / 2493

页数：5

共 22 条

[1] Preparation of Au nanoparticles-coated polystyrene beads and its application in protein immobilization [J].

Cao, Yuan-Cheng ;

Wang, Zhan ;

Jin, Xin ;

Hua, Xiao-Feng ;

Liu, Man-Xi ;

Zhao, Yuan-Di .

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2009, 334 (1-3) :53-58

[2] Gold nanoelectrodes of varied size: Transition to molecule-like charging [J].

Chen, SW ;

Ingram, RS ;

Hostetler, MJ ;

Pietron, JJ ;

Murray, RW ;

Schaaff, TG ;

Khoury, JT ;

Alvarez, MM ;

Whetten, RL .

SCIENCE, 1998, 280 (5372) :2098-2101

[3] A glucose biosensor based on enzyme entrapment within polypyrrole films electrodeposited on mesoporous titanium dioxide [J].

Cosnier, S ;

Senillou, A ;

Grätzel, M ;

Comte, P ;

Vlachopoulos, N ;

Renault, NJ ;

Martelet, C .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1999, 469 (02) :176-181

[4] Biotinylated alginate immobilization matrix in the construction of an amperometric biosensor: application for the determination of glucose [J].

Cosnier, S ;

Novoa, A ;

Mousty, C ;

Marks, RS .

ANALYTICA CHIMICA ACTA, 2002, 453 (01) :71-79

[5] Dendrimers as nanoreactors to produce platinum nanoparticles embedded in layer-by-layer films for methanol-tolerant cathodes [J].

Crespilho, FN ;

Huguenin, F ;

Zucolotto, V ;

Olivi, P ;

Nart, FC ;

Oliveira, ON .

ELECTROCHEMISTRY COMMUNICATIONS, 2006, 8 (02) :348-352

[6] Gold nanoparticles: Assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology [J].

Daniel, MC ;

Astruc, D .

CHEMICAL REVIEWS, 2004, 104 (01) :293-346

[7] Mussel-inspired surface chemistry for multifunctional coatings [J].

Lee, Haeshin ;

Dellatore, Shara M. ;

Miller, William M. ;

Messersmith, Phillip B. .

SCIENCE, 2007, 318 (5849) :426-430

[8] A novel glucose biosensor based on immobilization of glucose oxidase into multiwall carbon nanotubes-polyelectrolyte-loaded electrospun nanofibrous membrane [J].

Manesh, K. M. ;

Kim, Hyun Tae ;

Santhosh, P. ;

Gopalan, A. I. ;

Lee, Kwang-Pill .

BIOSENSORS & BIOELECTRONICS, 2008, 23 (06) :771-779

[9] A microdialysis technique for continuous subcutaneous glucose monitoring in diabetic patients (part 1) [J].

Poscia, A ;

Mascini, M ;

Moscone, D ;

Luzzana, M ;

Caramenti, G ;

Cremonesi, P ;

Valgimigli, F ;

Bongiovanni, C ;

Varalli, M .

BIOSENSORS & BIOELECTRONICS, 2003, 18 (07) :891-898

[10] Electrochemical resolution of 15 oxidation states for monolayer protected gold nanoparticles [J].

Quinn, BM ;

Liljeroth, P ;

Ruiz, V ;

Laaksonen, T ;

Kontturi, K .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (22) :6644-6645

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