Crystallization and preliminary X-ray diffraction analysis of the azoreductase PpAzoR from Pseudomonas putida MET94

被引：5

作者：

Correia, Bruno ^{[1
]}

Chen, Zhenjia ^{[1
]}

Mendes, Sonia ^{[1
]}

Martins, Ligia O. ^{[1
]}

Bento, Isabel ^{[1
]}

机构：

[1] Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2780257 Oeiras, Portugal

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2011年 / 67卷

关键词：

MOLECULAR-CLONING; AZO DYES; METABOLISM; REDUCTASE;

D O I：

10.1107/S1744309110048220

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

PpAzoR, an FMN-dependent NADPH azoreductase from Pseudomonas putida MET94, has been crystallized using the sitting-drop vapour-diffusion technique. The crystals diffracted to 1.6 angstrom resolution using synchrotron radiation and belonged to the orthorhombic space group F222, with unit-cell parameters a = 72.1, b = 95.5, c = 146.1 angstrom. Data sets were collected from the native protein to 2.2 angstrom resolution using in-house equipment and to 1.6 angstrom resolution using synchrotron radiation and the three-dimensional structure was determined by the molecular-replacement method.

引用

页码：121 / 123

页数：3

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