Detection of DNA of six human herpesviruses in the cerebrospinal fluid of immunocompetent non-herpetic acute limbic encephalitis patients

被引：5

作者：

Yoshikawa, Tetsushi ^{[1
]}

Asano, Yoshizo ^{[1
]}

Takahashi, Yukitoshi ^{[2
]}

机构：

[1] Fujita Hlth Univ, Sch Med, Dept Pediat, Toyoake, Aichi 4701192, Japan

[2] Shizuoka Inst Epilepsy & Neurol Disorder, Dept Res, Natl Epilepsy Ctr, Shizuoka, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2010年 / 54卷 / 08期

关键词：

limbic encephalitis; real-time PCR; EBV; REAL-TIME PCR; CENTRAL-NERVOUS-SYSTEM; VIRAL LOAD; QUANTITATIVE-ANALYSIS; CELL TRANSPLANTATION; HUMAN-HERPESVIRUS-6; VIRUS; SPECIMENS; ASSAY;

D O I：

10.1111/j.1348-0421.2010.00234.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In order to determine whether six other human herpesviruses, aside from herpes simplex virus, are associated with non-herpetic acute limbic encephalitis in immunocompetent individuals, real-time PCR was used to detect the DNA of herpesviruses in CSF collected from 61 patients with this form of encephalitis. Five of the human herpesviruses tested were not detected in any of the 61 CSF samples. EBV DNA was detected in one CSF sample. The EBV DNA-positive patient was a 36-year-old woman who presented with fever, headache, mild somnolence, and the typical neuroimaging findings.

引用

页码：471 / 474

页数：4

共 18 条

[1] NEUROLOGIC COMPLICATIONS OF INFECTIOUS-MONONUCLEOSIS [J].

CONNELLY, KP ;

DEWITT, LD .

PEDIATRIC NEUROLOGY, 1994, 10 (03) :181-184

[2] HHV-6 variant a meningoencephalitis after allogeneic hematopoietic stem cell transplantation diagnosed by quantitative real-time polymerase chain reaction [J].

Gauthert-Dejean, A ;

Bossi, P ;

Vernant, JP ;

Dhedin, N .

TRANSPLANTATION, 2005, 80 (04) :539-539

[3] Paraneoplastic limbic encephalitis: neurological symptoms, immunological findings and tumour association in 50 patients [J].

Gultekin, SH ;

Rosenfeld, MR ;

Voltz, R ;

Eichen, J ;

Posner, JB ;

Dalmau, J .

BRAIN, 2000, 123 :1481-1494

[4] Evidence of human herpesvirus 6 infection in 4 immunocompetent patients with encephalitis [J].

Isaacson, E ;

Glaser, CA ;

Forghani, B ;

Amad, Z ;

Wallace, M ;

Armstrong, RW ;

Exner, MM ;

Schmid, S .

CLINICAL INFECTIOUS DISEASES, 2005, 40 (06) :890-893

[5]

Kimura H, 2000, J CLIN MICROBIOL, V38, P2447

[6] Quantitative analysis of Epstein-Barr virus load by using a real-time PCR assay [J].

Kimura, H ;

Morita, M ;

Yabuta, Y ;

Kuzushima, K ;

Kato, K ;

Kojima, S ;

Matsuyama, T ;

Morishima, T .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (01) :132-136

[7] Comparison of a LightCycler-based real-time PCR for quantitation of Epstein-Barr viral load in different clinical specimens with semiquantitative PCR [J].

Krumbholz, A ;

Meerbach, A ;

Zell, R ;

Gruhn, B ;

Henke, A ;

Birch-Hirschfeld, E ;

Wutzler, P .

JOURNAL OF MEDICAL VIROLOGY, 2006, 78 (05) :598-607

[8] Quantitative analysis of human herpesvirus 8 viral load using a real-time PCR assay [J].

Lallemand, F ;

Desire, N ;

Rozenbaum, W ;

Nicolas, JC ;

Marechal, V .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (04) :1404-1408

[9] Acute limbic encephalitis: A new entity? [J].

Mochizuki, Y ;

Mizutani, T ;

Isozaki, E ;

Ohtake, T ;

Takahashi, Y .

NEUROSCIENCE LETTERS, 2006, 394 (01) :5-8

[10] Real-time polymerase chain reaction detection of herpes simplex virus in cerebrospinal fluid and cost savings from earlier hospital discharge [J].

Rand, K ;

Houck, H ;

Lawrence, R .

JOURNAL OF MOLECULAR DIAGNOSTICS, 2005, 7 (04) :511-516

← 1 2 →