Molecular cloning and sequence analysis of canine acidic ribosomal phosphoprotein P0 cDNA

被引：0
作者：
Canatan, H
Lin, YC
机构：
[1] Ohio State Univ, Ctr Comprehens Canc, Columbus, OH 43210 USA
[2] Ohio State Univ, Coll Vet Med, Lab Reprod & Mol Endocrinol, Columbus, OH 43210 USA
[3] Ohio State Univ, Coll Biol Sci, Mol Cellular & Dev Biol Program, Columbus, OH 43210 USA
来源：
DNA SEQUENCE | 2000年 / 11卷 / 3-4期
关键词：
acidic ribosomal phosphoprotein P0; canine; cDNA; cloning; prostate;
D O I：
10.3109/10425170009033250
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Acidic ribosomal phosphoprotein PO has been widely used as an estradiol-independent housekeeping gene. Expression of acidic ribosomal phosphoprotein PO in canine prostate was detected and canine specific cDNA was cloned. Total cellular RNA was isolated from normal young (5 month-old) canine prostate tissue. Total cDNAs were synthesized from total cellular RNAs by reverse transcription assay. Primers used in polymerase chain reaction were designed from published human ARP0 cDNA sequences and utilized to amplify 562 base-pair (bp) canine acidic ribosomal phosphoprotein PO cDNA. Nucleotide sequences of canine ARP0 cDNA clones were determined on both strands. Canine ARP0 cDNA and their deduced amino acid sequences share very high homology to ARP0 orthologs from other vertebrate species including human, mouse, rat, chicken, and bovine.
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页码：321 / 326
页数：6
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