Genome-Wide Analysis of MYB Gene Family in Chinese Bayberry (Morella rubra) and Identification of Members Regulating Flavonoid Biosynthesis

被引:47
作者
Cao, Yunlin [1 ,2 ,3 ]
Jia, Huimin [3 ]
Xing, Mengyun [1 ,2 ,3 ]
Jin, Rong [4 ]
Grierson, Donald [1 ,2 ,3 ,5 ]
Gao, Zhongshan [3 ]
Sun, Chongde [1 ,2 ,3 ]
Chen, Kunsong [1 ,2 ,3 ]
Xu, Changjie [1 ,2 ,3 ]
Li, Xian [1 ,2 ,3 ]
机构
[1] Zhejiang Univ, Zhejiang Prov Key Lab Hort Plant Integrat Biol, Hangzhou, Peoples R China
[2] Zhejiang Univ, State Agr Minist Lab Hort Plant Growth, Dev & Qual Improvement, Hangzhou, Peoples R China
[3] Zhejiang Univ, Inst Fruit Sci, Coll Agr & Biotechnol, Hangzhou, Peoples R China
[4] Zhejiang Univ, Agr Expt Stn, Hangzhou, Peoples R China
[5] Univ Nottingham, Sch Biosci, Plant & Crop Sci Div, Loughborough, Leics, England
基金
中国国家自然科学基金;
关键词
Chinese bayberry; MYB transcription factors; transcriptional regulation; anthocyanins; flavonols; proanthocyanidins; flavonoid biosynthesis; R2R3-MYB TRANSCRIPTION FACTOR; MYRICA-RUBRA; PROANTHOCYANIDIN BIOSYNTHESIS; ANTHOCYANIN BIOSYNTHESIS; EXPRESSION ANALYSIS; ARABIDOPSIS; GRAPEVINE; EVOLUTIONARY; ZUCC; SIEB;
D O I
10.3389/fpls.2021.691384
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Chinese bayberry (Morella rubra), the most economically important fruit tree in the Myricaceae family, is a rich source of natural flavonoids. Recently the Chinese bayberry genome has been sequenced, and this provides an opportunity to investigate the organization and evolutionary characteristics of MrMYB genes from a whole genome view. In the present study, we performed the genome-wide analysis of MYB genes in Chinese bayberry and identified 174 MrMYB transcription factors (TFs), including 122 R2R3-MYBs, 43 1R-MYBs, two 3R-MYBs, one 4R-MYB, and six atypical MYBs. Collinearity analysis indicated that both syntenic and tandem duplications contributed to expansion of the MrMYB gene family. Analysis of transcript levels revealed the distinct expression patterns of different MrMYB genes, and those which may play important roles in leaf and flower development. Through phylogenetic analysis and correlation analyses, nine MrMYB TFs were selected as candidates regulating flavonoid biosynthesis. By using dual-luciferase assays, MrMYB12 was shown to trans-activate the MrFLS1 promoter, and MrMYB39 and MrMYB58a trans-activated the MrLAR1 promoter. In addition, overexpression of 35S:MrMYB12 caused a significant increase in flavonol contents and induced the expression of NtCHS, NtF3H, and NtFLS in transgenic tobacco leaves and flowers and significantly reduced anthocyanin accumulation, resulting in pale-pink or pure white flowers. This indicates that MrMYB12 redirected the flux away from anthocyanin biosynthesis resulting in higher flavonol content. The present study provides valuable information for understanding the classification, gene and motif structure, evolution and predicted functions of the MrMYB gene family and identifies MYBs regulating different aspects of flavonoid biosynthesis in Chinese bayberry.
引用
收藏
页数:15
相关论文
共 57 条
[1]   MdMYB9 and MdMYB11 are Involved in the Regulation of the JA-Induced Biosynthesis of Anthocyanin and Proanthocyanidin in Apples [J].
An, Xiu-Hong ;
Tian, Yi ;
Chen, Ke-Qin ;
Liu, Xiao-Juan ;
Liu, Dan-Dan ;
Xie, Xing-Bin ;
Cheng, Cun-Gang ;
Cong, Pei-Hua ;
Hao, Yu-Jin .
PLANT AND CELL PHYSIOLOGY, 2015, 56 (04) :650-662
[2]   Biochemical and Molecular Analysis of Pink Tomatoes: Deregulated Expression of the Gene Encoding Transcription Factor S1MYB12 Leads to Pink Tomato Fruit Color [J].
Ballester, Ana-Rosa ;
Molthoff, Jos ;
de Vos, Ric ;
Hekkert, Bas te Lintel ;
Orzaez, Diego ;
Fernandez-Moreno, Josefina-Patricia ;
Tripodi, Pasquale ;
Grandillo, Silvana ;
Martin, Cathie ;
Heldens, Jos ;
Ykema, Marieke ;
Granell, Antonio ;
Bovy, Arnaud .
PLANT PHYSIOLOGY, 2010, 152 (01) :71-84
[3]   Control of cell and petal morphogenesis by R2R3 MYB transcription factors [J].
Baumann, Kim ;
Perez-Rodriguez, Maria ;
Bradley, Desmond ;
Venail, Julien ;
Bailey, Paul ;
Jin, Hailing ;
Koes, Ronald ;
Roberts, Keith ;
Martin, Cathie .
DEVELOPMENT, 2007, 134 (09) :1691-1701
[4]   The grapevine transcription factor VvMYBPA1 regulates proanthocyanidin synthesis during fruit development [J].
Bogs, Jochen ;
Jaffe, Felix W. ;
Takos, Adam M. ;
Walker, Amanda R. ;
Robinson, Simon P. .
PLANT PHYSIOLOGY, 2007, 143 (03) :1347-1361
[5]   The roles of segmental and tandem gene duplication in the evolution of large gene families in Arabidopsis thaliana [J].
Cannon S.B. ;
Mitra A. ;
Baumgarten A. ;
Young N.D. ;
May G. .
BMC Plant Biology, 4 (1)
[6]   PpMYB15 and PpMYBF1 Transcription Factors Are Involved in Regulating Flavonol Biosynthesis in Peach Fruit [J].
Cao, Yunlin ;
Xie, Linfeng ;
Ma, Yingyue ;
Ren, Chuanhong ;
Xing, Mengyun ;
Fu, Zishan ;
Wu, Xinyue ;
Yin, Xueren ;
Xu, Changjie ;
Li, Xian .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2019, 67 (02) :644-652
[7]   MYB Transcription Factors in Chinese Pear (Pyrus bretschneideri Rehd.): Genome-Wide Identification, Classification, and Expression Profiling during Fruit Development [J].
Cao, Yunpeng ;
Han, Yahui ;
Li, Dahui ;
Lin, Yi ;
Cai, Yongping .
FRONTIERS IN PLANT SCIENCE, 2016, 7
[8]   TBtools: An Integrative Toolkit Developed for Interactive Analyses of Big Biological Data [J].
Chen, Chengjie ;
Chen, Hao ;
Zhang, Yi ;
Thomas, Hannah R. ;
Frank, Margaret H. ;
He, Yehua ;
Xia, Rui .
MOLECULAR PLANT, 2020, 13 (08) :1194-1202
[9]   The MYB transcription factor superfamily of arabidopsis: Expression analysis and phylogenetic comparison with the rice MYB family [J].
Chen, YH ;
Yang, XY ;
He, K ;
Liu, MH ;
Li, JG ;
Gao, ZF ;
Lin, ZQ ;
Zhang, YF ;
Wang, XX ;
Qiu, XM ;
Shen, YP ;
Zhang, L ;
Deng, XH ;
Luo, JC ;
Deng, XW ;
Chen, ZL ;
Gu, HY ;
Qu, LJ .
PLANT MOLECULAR BIOLOGY, 2006, 60 (01) :107-124
[10]   Multiple sequence alignment with the Clustal series of programs [J].
Chenna, R ;
Sugawara, H ;
Koike, T ;
Lopez, R ;
Gibson, TJ ;
Higgins, DG ;
Thompson, JD .
NUCLEIC ACIDS RESEARCH, 2003, 31 (13) :3497-3500