Fluorescence correlation spectroscopy for probing the kinetics and mechanisms of DNA hairpin formation

被引：46

作者：

Van Orden, Alan ^{[1
]}

Jung, Jaemyeong ^{[1
]}

机构：

[1] Colorado State Univ, Dept Chem, Ft Collins, CO 80523 USA

来源：

BIOPOLYMERS | 2008年 / 89卷 / 01期

关键词：

autocorrelation; cross-correlation; DNA hairpin;

D O I：

10.1002/bip.20826

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This article reviews the application of fluorescence correlation spectroscopy (FCS) and related techniques to the study. of nucleic acid hairpin conformational fluctuations in free aqueous solutions. Complimentary results obtained using laser-induced temperature jump spectroscopy, single-molecule fluorescence spectroscopy, optical trapping, and biophysical theory are also discussed. The studies cited reveal that DNA and RNA hairpin folding occurs by way of a complicated reaction mechanism involving long- and short-lived reaction intermediates. Reactions occurring on the subnanoseconds to seconds time scale have been observed, pointing out the need for experimental techniques capable of probing a broad range of reaction times in the study of such complex, multistate reactions. (c) 2007 Wiley Periodicals, Inc.

引用

页码：1 / 16

页数：16

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