Kinetic Studies on CphA Mutants Reveal the Role of the P158-P172 Loop in Activity versus Carbapenems

被引：12

作者：

Bottoni, Carlo ^{[1
]}

Perilli, Mariagrazia ^{[1
]}

Marcoccia, Francesca ^{[1
]}

Piccirilli, Alessandra ^{[1
]}

Pellegrini, Cristina ^{[1
]}

Colapietro, Martina ^{[1
]}

Sabatini, Alessia ^{[1
]}

Celenza, Giuseppe ^{[1
]}

Kerff, Frederic ^{[2
]}

Amicosante, Gianfranco ^{[1
]}

Galleni, Moreno ^{[2
]}

Mercuri, Paola Sandra ^{[2
]}

机构：

[1] Univ Aquila, Dipartimento Sci Clin Appl & Biotecnol, I-67100 Laquila, Italy

[2] Univ Liege, Inst Chim B6, CIP, Macromol Biol, Liege, Belgium

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2016年 / 60卷 / 05期

关键词：

METALLO-BETA-LACTAMASE; AEROMONAS-HYDROPHILA AE036; SFH-I;

D O I：

10.1128/AAC.01703-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Site-directed mutagenesis of CphA indicated that prolines in the P158-P172 loop are essential for the stability and the catalytic activity of subclass B2 metallo-beta-lactamases against carbapenems. The sequential substitution of proline led to a decrease of the catalytic efficiency of the variant compared to the wild-type (WT) enzyme but also to a higher affinity for the binding of the second zinc ion.

引用

页码：3123 / 3126

页数：4

共 8 条

[1] The Structure of the Dizinc Subclass B2 Metallo-β-Lactamase CphA Reveals that the Second Inhibitory Zinc Ion Binds in the Histidine Site [J].

Bebrone, Carine ;

Delbrueck, Heinrich ;

Kupper, Michael B. ;

Schloemer, Philipp ;

Willmann, Charlotte ;

Frere, Jean-Marie ;

Fischer, Rainer ;

Galleni, Moreno ;

Hoffmann, Kurt M. V. .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (10) :4464-4471

[2] THE 3-D STRUCTURE OF A ZINC METALLO-BETA-LACTAMASE FROM BACILLUS-CEREUS REVEALS A NEW-TYPE OF PROTEIN FOLD [J].

CARFI, A ;

PARES, S ;

DUEE, E ;

GALLENI, M ;

DUEZ, C ;

FRERE, JM ;

DIDEBERG, O .

EMBO JOURNAL, 1995, 14 (20) :4914-4921

[3]

Clinical and Laboratory Standards Institute, 2012, M09A7 CLSI

[4] Biochemical Characterization of Sfh-I, a Subclass B2 Metallo-β-Lactamase from Serratia fonticola UTAD54 [J].

Fonseca, Fatima ;

Arthur, Christopher J. ;

Bromley, Elizabeth H. C. ;

Samyn, Bart ;

Moerman, Pablo ;

Saavedra, Maria Jose ;

Correia, Antonio ;

Spencer, James .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2011, 55 (11) :5392-5395

[5] Crystal Structure of Serratia fonticola Sfh-I: Activation of the Nucleophile in Mono-Zinc Metallo-β-Lactamases [J].

Fonseca, Fatima ;

Bromley, Elizabeth H. C. ;

Saavedra, Maria Jose ;

Correia, Antonio ;

Spencer, James .

JOURNAL OF MOLECULAR BIOLOGY, 2011, 411 (05) :951-959

[6] Update of the standard numbering scheme for class B β-lactamases [J].

Garau, G ;

García-Sáez, I ;

Bebrone, C ;

Anne, C ;

Mercuri, P ;

Galleni, M ;

Frère, JM ;

Dideberg, O .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (07) :2347-2349

[7] HIGH SPECIFICITY OF CPHA-ENCODED METALLO-BETA-LACTAMASE FROM AEROMONAS-HYDROPHILA AE036 FOR CARBAPENEMS AND ITS CONTRIBUTION TO BETA-LACTAM RESISTANCE [J].

SEGATORE, B ;

MASSIDDA, O ;

SATTA, G ;

SETACCI, D ;

AMICOSANTE, G .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1993, 37 (06) :1324-1328

[8] Zn(II) dependence of the Aeromonas hydrophila AE036 metallo-beta-lactamase activity and stability [J].

Valladares, MH ;

Felici, A ;

Weber, G ;

Adolph, HW ;

Zeppezauer, M ;

Rossolini, GM ;

Amicosante, G ;

Frere, JM ;

Galleni, M .

BIOCHEMISTRY, 1997, 36 (38) :11534-11541

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