RNA interference-madiated gene silencing of vascular endothelial growth factor in colon cancer cells

被引：4
作者：
Li, Tie-Jun
Song, Jian-Ning
Kang, Kai
Tong, Shu-Sheng
Hu, Zan-Lan
He, Tong-Chuan
Zhang, Bing-Qiang
Zhang, Cai-Quan [1 ]
机构：
[1] Chongqing Univ Med Sci, Affiliated Hosp 1, Dept Gen Surg, Chongqing 400016, Peoples R China
[2] Ninth Hosp Chongqing, Chongqing 400700, Peoples R China
来源：
WORLD JOURNAL OF GASTROENTEROLOGY | 2007年 / 13卷 / 40期
关键词：
RNA interference; vascular endothelial growth factor; colon cancer; northern blotting; western; blotting;
D O I：
10.3748/wjg.v13.i40.5312
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
AIM: To inhibit the expression of vascular endothelial growth factor (VEGF) in colon cancer cell line by RNA interference (RNAi). METHODS: Followed the service of E-RNAi, we designed and constructed two kinds of shRNA expression vectors aiming at the VEGF gene, then transfected them into colon cancer HT29 cells by lipofectamine (TM) 2000. The level of VEGF mRNA was investigated by RT-PCR and Northern blotting. The protein expression of VEGF was observed by immunofluoresence staining and Western blotting. RESULTS: We got two kinds of VEGF specific shRNA expression vectors which could efficiently inhibit the expression of VEGF in HT29 cells. RT-PCR, Northern blotting, immunofluoresence staining and Western blotting showed that inhibition rate for VEGF expression was up to 42%, 89%, 73% and 82%, respectively. CONCLUSION: The expression of VEGF can be inhibited by RNA interference in HT29 cells.
引用
收藏
页码：5312 / 5316
页数：5
相关论文
共 32 条
[1]   RNAi and related mechanisms and their potential use for therapy [J].
Agami, R .
CURRENT OPINION IN CHEMICAL BIOLOGY, 2002, 6 (06) :829-834
[2]   RNA interference may be more potent than antisense RNA in human cancer cell lines [J].
Aoki, Y ;
Cioca, DP ;
Oidaira, H ;
Kamiya, J ;
Kiyosawa, K .
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 2003, 30 (1-2) :96-102
[3]   VEGF upregulates BCL-2 expression and is associated with decreased apoptosis in neuroblastoma cells [J].
Beierle, EA ;
Strande, LF ;
Chen, MK .
JOURNAL OF PEDIATRIC SURGERY, 2002, 37 (03) :467-471
[4]   Role for a bidentate ribonuclease in the initiation step of RNA interference [J].
Bernstein, E ;
Caudy, AA ;
Hammond, SM ;
Hannon, GJ .
NATURE, 2001, 409 (6818) :363-366
[5]   The efficacy of small interfering RNAs targeted to the type 1 insulin-like growth factor receptor (IGF1R) is influenced by secondary structure in the IGF1R transcript [J].
Bohula, EA ;
Salisbury, AJ ;
Sohail, M ;
Playford, MP ;
Riedemann, J ;
Southern, EM ;
Macaulay, VM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (18) :15991-15997
[6]   A system for stable expression of short interfering RNAs in mammalian cells [J].
Brummelkamp, TR ;
Bernards, R ;
Agami, R .
SCIENCE, 2002, 296 (5567) :550-553
[7]   RNA interference is a functional pathway with therapeutic potential in human myeloid leukemia cell lines [J].
Cioca, DP ;
Aoki, Y ;
Kiyosawa, K .
CANCER GENE THERAPY, 2003, 10 (02) :125-133
[8]   VEGF165 promotes survival of leukemic cells by Hsp90-mediated induction of Bcl-2 expression and apoptosis inhibition [J].
Dias, S ;
Shmelkov, SV ;
Lam, G ;
Rafii, S .
BLOOD, 2002, 99 (07) :2532-2540
[9]   Overexpression and significance of prion protein in gastric cancer and multidrug-resistant gastric carcinoma cell line SGC7901/ADR [J].
Du, JP ;
Pan, YL ;
Shi, YQ ;
Guo, CC ;
Jin, XH ;
Sun, L ;
Liu, N ;
Qiao, T ;
Fan, DM .
INTERNATIONAL JOURNAL OF CANCER, 2005, 113 (02) :213-220
[10]   Knockdown of Rab25 expression by RNAi inhibits growth of human epithelial ovarian cancer cells in vitro and in vivo [J].
Fan Yang ;
Xin Xiao-Yan ;
Chen Bi-Liang ;
Ma Xiangdong .
PATHOLOGY, 2006, 38 (06) :561-567
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