Dental stem cell-conditioned medium for tissue regeneration: Optimization of production and storage

被引:11
作者
Chouaib, Batoul [1 ]
Cuisinier, Frederic [1 ]
Collart-Dutilleul, Pierre-Yves [1 ,2 ]
机构
[1] Univ Montpellier, Lab Bioengn, Nanosci UR UM104, F-34000 Montpellier, France
[2] Univ Montpellier, Lab Bioengn, Nanosci UR UM104, 545 Ave Prof Jean-Louis Viala, F-34000 Montpellier, France
来源
WORLD JOURNAL OF STEM CELLS | 2022年 / 14卷 / 04期
关键词
Tissue engineering; Mesenchymal stem cells; Dental; Conditioned medium; Secretome; Regeneration; SHOW MULTIFACETED BENEFITS; SIDE POPULATION CELLS; RAT SPINAL-CORD; COLONY-STIMULATING FACTOR; IG-LIKE LECTIN-9; BONE-MARROW; ODONTOGENIC DIFFERENTIATION; THERAPEUTIC BENEFITS; SECRETED ECTODOMAIN; MULTIPOTENT CELLS;
D O I
10.4252/wjsc.v14.i4.287
中图分类号
Q813 [细胞工程];
学科分类号
摘要
BACKGROUND Mesenchymal stem cells (MSC) effects on tissue regeneration are mainly mediated by their secreted substances (secretome), inducing their paracrine activity. This Conditioned medium (CM), including soluble factors (proteins, nucleic acids, lipids) and extracellular vesicles is emerging as a potential alternative to cell therapy. However, the manufacturing of CM suffers from variable procedures and protocols leading to varying results between studies. Besides, there is no well-defined optimized procedure targeting specific applications in regenerative medicine. AIM To focus on conditioned medium produced from dental MSC (DMSC-CM), we reviewed the current parameters and manufacturing protocols, in order to propose a standardization and optimization of these manufacturing procedures. METHODS We have selected all publications investigating the effects of dental MSC secretome in in vitro and in vivo models of tissue regeneration, in accordance with the PRISMA guidelines. RESULTS A total of 351 results were identified. And based on the inclusion criteria described above, 118 unique articles were included in the systematic review. DMSC-CM production was considered at three stages: before CM recovery (cell sources for CM), during CM production (culture conditions) and after production (CM treatment). CONCLUSION No clear consensus could be recovered as evidence-based methods, but we were able to describe the most commonly used protocols: donors under 30 years of age, dental pulp stem cells and exfoliated deciduous tooth stem cells with cell passage between 1 and 5, at a confluence of 70% to 80%. CM were often collected during 48 h, and stored at -80 ?. It is important to point out that the preconditioning environment had a significant impact on DMSC-CM content and efficiency. (C) The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved
引用
收藏
页码:287 / 302
页数:16
相关论文
共 140 条
[1]   A compound of concentrated growth factor and periodontal ligament stem cell-derived conditioned medium [J].
Aghamohamadi, Z. ;
Kadkhodazadeh, M. ;
Torshabi, M. ;
Tabatabaei, F. .
TISSUE & CELL, 2020, 65
[2]   Therapeutic Potential of Dental Pulp Stem Cell Secretome for Alzheimer's Disease Treatment: An In Vitro Study [J].
Ahmed, Nermeen El-Moataz Bellah ;
Murakami, Masashi ;
Hirose, Yujiro ;
Nakashima, Misako .
STEM CELLS INTERNATIONAL, 2016, 2016
[3]   Recruitment of mesenchymal stem cells by stromal cell-derived factor 1α in pulp cells from deciduous teeth [J].
Akazawa, Yuki ;
Hasegawa, Tomokazu ;
Yoshimura, Yoshitaka ;
Chosa, Naoyuki ;
Asakawa, Takeyoshi ;
Ueda, Kimiko ;
Sugimoto, Asuna ;
Kitamura, Takamasa ;
Nakagawa, Hiroshi ;
Ishisaki, Akira ;
Iwamoto, Tsutomu .
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2015, 36 (02) :442-448
[4]  
Alkhalil Moustafa, 2015, Med Glas (Zenica), V12, P27
[5]   Variation in human dental pulp stem cell ageing profiles reflect contrasting proliferative and regenerative capabilities [J].
Alraies, Amr ;
Alaidaroos, Nadia Y. A. ;
Waddington, Rachel J. ;
Moseley, Ryan ;
Sloan, Alastair J. .
BMC CELL BIOLOGY, 2017, 18
[6]   Hypoxia Enhances the Angiogenic Potential of Human Dental Pulp Cells [J].
Aranha, Andreza M. F. ;
Zhang, Zhaocheng ;
Neiva, Kathleen G. ;
Costa, Carlos A. S. ;
Hebling, Josimeri ;
Noer, Jacques E. .
JOURNAL OF ENDODONTICS, 2010, 36 (10) :1633-1637
[7]   Sensory and Motor Behavior Evidences Supporting the Usefulness of Conditioned Medium from Dental Pulp-Derived Stem Cells in Spinal Cord Injury in Rats [J].
Asadi-Golshan, Reza ;
Razban, Vahid ;
Mirzaei, Esmaeil ;
Rahrnanian, Abdolkarim ;
Khajeh, Sahar ;
Mostafavi-Pour, Zohreh ;
Dehghani, Farzaneh .
ASIAN SPINE JOURNAL, 2018, 12 (05) :785-793
[8]   Effect of Different Preconditioning Regimens on the Expression Profile of Murine Adipose-Derived Stromal/Stem Cells [J].
Baer, Patrick C. ;
Overath, Juergen M. ;
Urbschat, Anja ;
Schubert, Ralf ;
Koch, Benjamin ;
Bohn, Asanke A. ;
Geiger, Helmut .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2018, 19 (06)
[9]   Angiogenic Potential and Secretome of Human Apical Papilla Mesenchymal Stem Cells in Various Stress Microenvironments [J].
Bakopoulou, Athina ;
Kritis, Aristeidis ;
Andreadis, Dimitrios ;
Papachristou, Eleni ;
Leyhausen, Gabriele ;
Koidis, Petros ;
Geurtsen, Werner ;
Tsiftsoglou, Asterios .
STEM CELLS AND DEVELOPMENT, 2015, 24 (21) :2496-2512
[10]   Angiogenic Properties of Human Dental Pulp Stem Cells [J].
Bronckaers, Annelies ;
Hilkens, Petra ;
Fanton, Yanick ;
Struys, Tom ;
Gervois, Pascal ;
Politis, Constantinus ;
Martens, Wendy ;
Lambrichts, Ivo .
PLOS ONE, 2013, 8 (08)