Measuring Hypopharyngeal Gland Acinus Size in Honey Bee (Apis mellifera) Workers

被引：24

作者：

Corby-Harris, Vanessa ^{[1
]}

Snyder, Lucy A. ^{[1
]}

机构：

[1] USDA ARS, Carl Hayden Bee Res Ctr, Washington, DC 20250 USA

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2018年 / 139期

关键词：

Environmental Sciences; Issue; 139; Apis mellifera; honey bee; hypopharyngeal gland; acini; jelly; Giemsa stain; nutrition;

D O I：

10.3791/58261

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The nurse hypopharyngeal glands produce the protein fraction of the worker and royal jelly that is fed to developing larvae and queens. These paired glands that are located in the head of the bee are highly sensitive to the quantity and quality of pollen and pollen substitutes that the nurse bee consumes. The glands get smaller when nurses are fed deficient diets and are large when they are fed complete diets. Because nurse hypopharyngeal gland size is a robust indicator of nurse nutrition, it is essential that those studying honey bee nutrition know how to measure these glands. Here, we provide detailed methods for dissecting, staining, imaging, and measuring nurse bee hypopharyngeal glands. We present comparisons of unstained and stained tissue and data that were used to study the impact of pollen on gland size. This method has been used to test how diet impacts hypopharyngeal gland size but has further use for understanding the role of these glands in hive health.

引用

页数：7

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