Restriction mapping in nanofluidic devices

被引：181

作者：

Riehn, R ^{[1
]}

Lu, MC

Wang, YM

Lim, SF

Cox, EC

Austin, RH

机构：

[1] Princeton Univ, Dept Phys, Princeton, NJ 08544 USA

[2] Princeton Univ, Dept Biol Mol, Princeton, NJ 08544 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2005年 / 102卷 / 29期

关键词：

D O I：

10.1073/pnas.0503809102

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have performed restriction mapping of DNA molecules using restriction endonucleases in nanochannels with diameters of 100-200 rim. The location of the restriction reaction within the device is controlled by electrophoresis and diffusion of Mg2+ and EDTA. We have successfully used the restriction enzymes Smal, Sad, and Pacl, and have been able to measure the positions of restriction sites with a precision of approximate to 1.5 kbp in 1 min using single DNA molecules.

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页码：10012 / 10016

页数：5

相关论文

共 20 条

[1] Single- and double-strand photocleavage of DNA by YO, YOYO and TOTO [J].

Akerman, B ;

Tuite, E .

NUCLEIC ACIDS RESEARCH, 1996, 24 (06) :1080-1090

[2] STRETCHING DNA WITH A RECEDING MENISCUS - EXPERIMENTS AND MODELS [J].

BENSIMON, D ;

SIMON, AJ ;

CROQUETTE, V ;

BENSIMON, A .

PHYSICAL REVIEW LETTERS, 1995, 74 (23) :4754-4757

[3] High-resolution restriction maps of bacterial artificial chromosomes constructed by optical mapping [J].

Cai, WW ;

Jing, JP ;

Irvin, B ;

Ohler, L ;

Rose, E ;

Shizuya, H ;

Kim, UJ ;

Simon, M ;

Anantharaman, T ;

Mishra, B ;

Schwartz, DC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (07) :3390-3395

[4] RAPID SIZING OF INDIVIDUAL FLUORESCENTLY STAINED DNA FRAGMENTS BY FLOW-CYTOMETRY [J].

GOODWIN, PM ;

JOHNSON, ME ;

MARTIN, JC ;

AMBROSE, WP ;

MARRONE, BL ;

JETT, JH ;

KELLER, RA .

NUCLEIC ACIDS RESEARCH, 1993, 21 (04) :803-806

[5] Bis-intercalation of a homodimeric thiazole orange dye in DNA in symmetrical pyrimidine-pyrimidine-purine-purine oligonucleotides [J].

Hansen, LF ;

Jensen, LK ;

Jacobsen, JP .

NUCLEIC ACIDS RESEARCH, 1996, 24 (05) :859-867

[6] Sacrificial polymers for nanofluidic channels in biological applications [J].

Li, WL ;

Tegenfeldt, JO ;

Chen, L ;

Austin, RH ;

Chou, SY ;

Kohl, PA ;

Krotine, J ;

Sturm, JC .

NANOTECHNOLOGY, 2003, 14 (06) :578-583

[7] Whole-genome shotgun optical mapping of Deinococcus radiodurans [J].

Lin, JY ;

Qi, R ;

Aston, C ;

Jing, JP ;

Anantharaman, TS ;

Mishra, B ;

White, O ;

Daly, MJ ;

Minton, KW ;

Venter, JC ;

Schwartz, DC .

SCIENCE, 1999, 285 (5433) :1558-1562

[8]

Madou MJ., 2002, FUNDAMENTALS MICROFA

[9] Inhibition of restriction endonuclease activity by DNA binding fluorochromes [J].

Meng, X ;

Cai, WW ;

Schwartz, DC .

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 1996, 13 (06) :945-951

[10]

Osoegawa K, 2000, GENOME RES, V10, P116