Novel alkenal/one reductase activity of yeast NADPH:quinone reductase Zta1p. Prospect of the functional role for the ζ-crystallin family

被引：12

作者：

Crosas, Eva ^{[1
]}

Porte, Sergio ^{[1
]}

Moeini, Agrin ^{[1
]}

Farres, Jaume ^{[1
]}

Biosca, Josep. A. ^{[1
]}

Pares, Xavier ^{[1
]}

Rosario Fernandez, M. ^{[1
]}

机构：

[1] Univ Autonoma Barcelona, Fac Biosci, Dept Biochem & Mol Biol, E-08193 Barcelona, Spain

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 2011年 / 191卷 / 1-3期

关键词：

Alkenal/one reductase; Quinone oxidoreductase; Double-bond alpha; beta-hydrogenation; Medium-chain dehydrogenases/reductases; zeta-Crystallin; ZTA1; OXIDOREDUCTASE; NADPH; IDENTIFICATION; INACTIVATION; PURIFICATION; INCREASE; PROTEIN; LENS;

D O I：

10.1016/j.cbi.2011.01.021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

zeta-Crystallins are a Zn2+-lacking enzyme group with quinone reductase activity, which belongs to the medium-chain dehydrogenase/reductase superfamily. It has been recently observed that human zeta-crystallin is capable of reducing the alpha,beta-double bond of alkenals and alkenones. Here we report that this activity is also shared by the homologous Zta1p enzyme from Saccharomyces cerevisiae. While the two enzymes show similar substrate specificity, human zeta-crystallin exhibits higher activity with lipid peroxidation products and Zta1p is more active with cinnamaldehyde. The presence of Zta1p has an in vivo protective effect on yeast strains exposed to the toxic substrate 3-penten-2-one. Analysis of ZTA1 gene expression indicates an induction under different types of cellular stress, including ethanol and dimethylsulfoxide exposure and by reaching the stationary growth phase. The role of Zta1p in the yeast adaptation to some stress types and the general functional significance of zeta-crystallins are discussed. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

引用

页码：32 / 37

页数：6

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