Molecular detection of equine trypanosomiasis in the Riyadh Province of Saudi Arabia

被引：9

作者：

Alanazi, Abdullah D. ^{[6
]}

Puschendorf, Robert ^{[1
]}

Salim, Bashir ^{[2
]}

Alyousif, Mohamed S. ^{[3
]}

Alanazi, Ibrahim O. ^{[4
]}

Al-shehri, Hajri R. ^{[5
]}

机构：

[1] Univ Plymouth, Sch Biol Sci, Plymouth, Devon, England

[2] Univ Khartoum, Dept Parasitol, Fac Vet Med, Khartoum, Sudan

[3] King Saud Univ, Dept Zool, Coll Sci, Riyadh, Saudi Arabia

[4] King Abdulaziz City Sci & Technol, Natl Biotechnol Ctr, Riyadh, Saudi Arabia

[5] Univ Liverpool Liverpool Sch Trop Med, Dept Parasitol, Liverpool, Merseyside, England

[6] Shaqra Univ, Fac Sci & Humanities, Dept Biol Sci, POB 1040, Ad Dawadimi 11911, Saudi Arabia

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2018年 / 30卷 / 06期

关键词：

Equine; ITS1; PCR; RoTat1.2; Saudi Arabia; trypanosomiasis; HORSES; PREVALENCE; DONKEYS; EVANSI; CAMELS; SURRA; PCR;

D O I：

10.1177/1040638718798688

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We conducted a cross-sectional study to detect trypanosome infections of horses and donkeys in the Riyadh Province of Saudi Arabia. DNA was extracted from blood samples collected from 368 horses and 142 donkeys, and subjected to universal first ribosomal internal transcribed spacer region (ITS1)-PCR followed by Trypanosoma evansi species-specific RoTat1.2-PCR. The universal ITS1-PCR revealed T. evansi infection in horses (n = 12; 3.3%) and donkeys (n = 4; 2.8%). There was no significant effect of sex or age on the prevalence of trypanosomiasis in horses or donkeys. Application of the RoTat1.2-PCR revealed that the RoTat1.2 VSG gene was absent from the positive ITS1-PCR samples of 3 horses and 1 donkey. This discrepancy could be explained by the circulation of T. evansi type B in Saudi Arabia; however, this suspicion requires confirmation.

引用

页码：942 / 945

页数：4

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