Rhizobium borbori sp. nov., aniline-degrading bacteria isolated from activated sludge

被引：36
作者：
Zhang, Guo Xia [1 ,2 ,3 ]
Ren, Sui Zhou [1 ,2 ]
Xu, Mei Ying [1 ,2 ]
Zeng, Guo Qu [1 ,2 ]
Luo, Hui Dong [1 ,2 ]
Chen, Jin Lin [1 ,2 ]
Tan, Zhi Yuan [3 ]
Sun, Guo Ping [1 ,2 ]
机构：
[1] Guangdong Inst Microbiol, Guangdong Open Lab Appl Microbiol, Guangzhou 510070, Guangdong, Peoples R China
[2] Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbial Culture Collect, Guangzhou 510070, Guangdong, Peoples R China
[3] S China Agr Univ, Coll Agr, Prov Key Lab Plant Mol Breeding, Guangzhou 510642, Guangdong, Peoples R China
来源：
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY | 2011年 / 61卷
关键词：
16S RIBOSOMAL-RNA; DELFTIA SP AN3; GENE-CLUSTER; DISCORDANT PHYLOGENIES; ROOT-NODULES; STRAIN YAA; DEGRADATION; IDENTIFICATION; CHINA; AGROBACTERIUM;
D O I：
10.1099/ijs.0.022228-0
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Three aniline-degrading bacteria, strains DN316(T), DN316-1 and DN365, were isolated from activated sludge. According to 16S rRNA gene sequence-based phylogenetic analysis, the isolates belonged to the genus Rhizobium, with Rhizobium (=Agrobacterium) radiobacter LMG 140(T) as the closest relative, with 96.5% sequence similarity. Phylogenetic analysis of the representative strain DN316(T) using sequences of the glnA, thrC and recA genes and the 16S-23S intergenic spacer region confirmed the phylogenetic arrangement obtained from analysis of the 16S rRNA gene. DNA-DNA relatedness between DN316(T) and R. radiobacter LMG 140(T) was 43.7%, clearly indicating that the representative strain DN316(T) represents a novel species. Phenotypic and biochemical characterization of the isolates and insertion sequence-PCR fingerprinting patterns showed several distinctive features that differentiated them from closely related species. The major components of the cellular fatty acids were C(18:1)omega 7c (57.10%), C(16:0) (11.31%) and C(19:0) cyclo omega 8c (10.13%). Based on our taxonomic analysis, the three isolates from activated sludge represent a novel species of the genus Rhizobium, for which the name Rhizobium borbofi sp. nov. is proposed. The type strain is DN316(T) (=CICC 10378(T) =LMG 23925(T)).
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收藏
页码：816 / 822
页数：7
相关论文
共 50 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]   Conjugal transfer of plasmid pNB2 to activated sludge bacteria leads to 3-chloroaniline degradation in enrichment cultures [J].
Bathe, S .
LETTERS IN APPLIED MICROBIOLOGY, 2004, 38 (06) :527-531
[3]   Rhizobium alamii sp nov., an exopolysaccharide-producing species isolated from legume and non-legume rhizospheres [J].
Berge, Odile ;
Lodhi, Asma ;
Brandelet, Geraldine ;
Santaella, Catherine ;
Roncato, Marie-Anne ;
Christen, Richard ;
Heulin, Thierry ;
Achouak, Wafa .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2009, 59 :367-372
[4]   Genetic diversity among 3-chloroaniline- and aniline-degrading strains of the Comamonadaceae [J].
Boon, N ;
Goris, J ;
De Vos, P ;
Verstraete, W ;
Top, EM .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (03) :1107-1115
[5]   Rhizobium hainanense sp. nov, isolated from tropical legumes [J].
Chen, WX ;
Tan, ZY ;
Gao, JL ;
Li, Y ;
Wang, ET .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1997, 47 (03) :870-873
[6]  
DELEY J, 1970, EUR J BIOCHEM, V12, P133
[7]   Rhizobial 16S rRNA and dnaK genes:: Mosaicism and the uncertain phylogenetic placement of Rhizobium galegae [J].
Eardly, BD ;
Nour, SA ;
van Berkum, P ;
Selander, RK .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (03) :1328-1335
[8]   Corresponding 16S rRNA gene segments in Rhizobiaceae and Aeromonas yield discordant phylogenies [J].
Eardly, BD ;
Wang, FS ;
vanBerkum, P .
PLANT AND SOIL, 1996, 186 (01) :69-74
[9]  
Frank B., 1889, Bet Dtsch Bot Ges, V7, P332, DOI [10.1111/j.1438-8677.1889.tb05711.x, DOI 10.1111/J.1438-8677.1889.TB05711.X]
[10]   Plasmid-encoded genes specifying aniline oxidation from Acinetobacter sp strain YAA [J].
Fujii, T ;
Takeo, M ;
Maeda, Y .
MICROBIOLOGY-UK, 1997, 143 :93-99
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