p16(INK4)/p15(INK4B) gene inactivation is a frequent event in malignant T-cell lines

被引:0
作者
Brandter, LB [1 ]
Heyman, M [1 ]
Rasool, O [1 ]
Liu, Y [1 ]
Grander, D [1 ]
Einhorn, S [1 ]
机构
[1] KAROLINSKA HOSP,RADIUMHEMMET,S-17176 STOCKHOLM,SWEDEN
关键词
tumor suppressor genes; p16(INK4)/p15(INK4B); T-cell lines;
D O I
暂无
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The cell cycle regulators p16(INK4) and p15(INK4B) have been mapped to the minimal region of overlap for chromosome 9p21 deletions, observed in a number of malignancies, suggesting that they could be tumor suppressor genes (TSGs). In the case of p16(INK4) this has been further substantiated by the finding of small intragenic mutations. In this study we have investigated the p16(INK4) and p15(INK4B) genes in 16 malignant T-cell lines by means of Southern blot, PCR and sequence analysis. p16(INK4) allelic deletions occurred in 15 of 16 cell lines; 12 of which were homozygous and 3 hemizygous. In 1 cell line (DND 41) the remaining p16(INK4) allele carried a microdeletion of 29 bp of exon 2, supporting the concept that p16(INK4) is a target TSG for deletions on 9p21. Most p16(INK4) deletions also included the p15(INK4B) gene. However, 4 of the cell lines deleted for p16(INK4) showed no evidence of p15(INK4B) loss, indicating that p15(INK4B) is not the target in these cell lines.
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页码:313 / 318
页数:6
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