Lipase-catalyzed synthesis of designer acylglycerols rich in residues of eicosapentaenoic, docosahexaenoic, conjugated linoleic, and/or stearic acids

被引：9

作者：

Torres, CF ^{[1
]}

Lin, B ^{[1
]}

Moeljadi, M ^{[1
]}

Hill, CG ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Chem Engn, Madison, WI 53706 USA

来源：

EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY | 2003年 / 105卷 / 10期

关键词：

Candida antarctica lipase; omega-3 fatty acids; conjugated linoleic acid; stearic acid; transesterification; glycerolysis;

D O I：

10.1002/ejlt.200300822

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Lipase-catalyzed glycerolysis of a commercial acylglycerol (TG500), rich in eicosapentaenoic residues, and subsequent reaction of the product acylglycerols via esterification and/or transesterification reactions were investigated for the purpose of preparing acylglycerols enriched in particular fatty acid residues. An immobilized lipase-from Candida antarctica (fraction B) was employed as biocatalyst for these reactions. The effects of enzyme loading, molar ratio of substrates, type of acyl donor, and use of a continuous purge stream of nitrogen were evaluated. The transesterification reactions were carried out using the ethyl esters of 3 different fatty acids, namely stearic, conjugated linoleic, and docosahexaenoic acids. Final yields of triacylglycerols of 75-80% were obtained for the transesterification reactions with the 3 different types of fatty acid ethyl esters.

引用

页码：614 / 623

页数：10

共 27 条

[1] One biocatalyst - Many applications: The use of Candida antarctica B-lipase in organic synthesis [J].