Optimization of bromelain isolation from pineapple byproducts by polysaccharide complex formation

被引：31

作者：

Campos, Debora A. ^{[1
]}

Coscueta, Ezequiel R. ^{[1
]}

Valetti, Nadia Woitovich ^{[2
,3
]}

Pastrana-Castro, Lorenzo M. ^{[4
]}

Teixeira, Jose A. ^{[5
]}

Pico, Guillermo A. ^{[2
,3
]}

Pintado, Maria Manuela ^{[1
]}

机构：

[1] Univ Catolica Portuguesa, CBQF, Lab Associado, Escola Super Biotecnol, Rua Arquiteto Lobao Vital 172, P-4200374 Porto, Portugal

[2] UNR, CONICET, IPROBYQ Biotechnol & Chem Proc Inst, Suipacha 570,S2002LRK, Rosario, Santa Fe, Argentina

[3] Natl Univ Rosario UNR, Coll Biochem & Pharmaceut Sci, Suipacha 570,S2002LRK, Rosario, Santa Fe, Argentina

[4] INL Int Iberian Nanotechnol Lab, P-4710330 Braga, Portugal

[5] Univ Minho, Ctr Engn Biol, Campus Gualtar, P-4710057 Braga, Portugal

来源：

FOOD HYDROCOLLOIDS | 2019年 / 87卷

关键词：

Protein complex; Natural crude juices; Carrageenan; Bromelain; BICINCHONINIC ACID; PURIFICATION; PRECIPITATION; WASTE;

D O I：

10.1016/j.foodhyd.2018.09.009

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A simple method for bromelain extraction from industrial pineapple residues (stems and peels) was developed and optimized through factorial experimental design. The developed methodology, based on precipitation with carrageenan, represents an alternative to the use of organic solvents and inorganic salts (common industrial precipitation) and allows achieving extracts with high bromelain purity. High recovery yield - 80-90% - of active bromelain was obtained for both crude juices (stems and peels) making possible to obtain ca. 0.3 g of bromelain from 100 g of pineapple byproducts using a low polysaccharide concentration (0.2-0.3% w/v).

引用

页码：792 / 804

页数：13

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