In-frame fusion of a His-Cys motif into the Pseudomonas aeruginosa outer membrane OprI lipoprotein results in increased metal binding capacity by Escherichia coli

被引：5

作者：

Bouia, A

Kholti, A

Saghi, M

Cornelis, P

机构：

[1] Free Univ Brussels VIB, Lab Microbial Interact, Dept Immunol Parasitol & Ultrastruct, B-1640 Rhode St Genese, Belgium

[2] Fac Sci Dhar Mehrez, Dept Biol, Fes, Morocco

来源：

RESEARCH IN MICROBIOLOGY | 2001年 / 152卷 / 09期

关键词：

surface display; metal binding; OprI; Pseudomonas aeruginosa; Escherichia coli;

D O I：

10.1016/S0923-2508(01)01263-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

OprI, a small outer membrane lipoprotein from Pseudomonas aeruginosa, can be produced in large amounts and anchored at the surface on Escherichia coli cells. A four-time repeated (His-Cys) motif was fused to the C-terminal part of OprI. After induction, E. coli cells harbouring the recombinant oprI gene became more sensitive to Cd and Co. The same cells, after IPTG induction, bound four to eight times more Cd and Cr than control cells expressing oprI alone. (C) 2001 Editions scientifiques et medicales Elsevier SAS.

引用

页码：799 / 804

页数：6

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