Enhanced photo-hydrogen production of Rhodopseudomonas faecalis RLD-53 by EDTA addition

被引：30

作者：

Ren, Hong-Yu ^{[1
]}

Liu, Bing-Feng ^{[1
]}

Ding, Jie ^{[1
]}

Nan, Jun ^{[1
]}

Xie, Guo-Jun ^{[1
]}

Zhao, Lei ^{[1
]}

Chen, Ming-Guang ^{[1
]}

Ren, Nan-Qi ^{[1
]}

机构：

[1] Harbin Inst Technol, State Key Lab Urban Water Resource & Environm, Harbin 150090, Peoples R China

来源：

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY | 2012年 / 37卷 / 10期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金; 中央高校基本科研业务费专项资金资助; 中国博士后科学基金;

关键词：

Hydrogen production; EDTA; Iron; Photo-fermentation; Nitrogenase activity; BIOHYDROGEN PRODUCTION; FERMENTATION; INHIBITION; GROWTH;

D O I：

10.1016/j.ijhydene.2012.02.071

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

This study investigated the effect of EDTA concentration in medium on the growth, hydrogen production and nitrogenase activity of Rhodopseudomonas faecalis RLD-53 by batch cultures. Experimental results indicated that bacterial growth and hydrogen production were strongly inhibited with EDTA concentration increasing to 0.6-0.7 g/L. However, the lag time of hydrogen production and the trends of biomass at EDTA concentration of 0-0.5 g/L were similar. The maximum cumulative hydrogen volume of 3325 ml H-2/L culture, hydrogen production rate of 27.6 ml H-2/L/h and hydrogen yield of 2.97 mol H-2/mol acetate were obtained when EDTA concentration was at 0.3 g/L, and the maximum OD660 attained 3.83. And the nitrogenase activity also reached a maximum value of 1331.9 mu l-C2H4/h/mg dry weight in the medium containing Fe2+ and EDTA. These results showed that a proper concentration of EDTA can promote the availability of iron, thereby further enhancing the activity of nitrogenase and photo-hydrogen production. Copyright (C) 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.

引用

页码：8277 / 8281

页数：5

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