An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity

被引：95

作者：

Huang, Jie ^{[1
]}

Gu, Lianfeng ^{[2
]}

Zhang, Ying ^{[1
]}

Yan, Tingxiu ^{[1
]}

Kong, Guanghui ^{[1
]}

Kong, Liang ^{[1
]}

Guo, Baodian ^{[1
,3
]}

Qiu, Min ^{[1
]}

Wang, Yang ^{[1
]}

Jing, Maofeng ^{[1
]}

Xing, Weiman ^{[3
]}

Ye, Wenwu ^{[1
,4
]}

Wu, Zhe ^{[5
]}

Zhang, Zhengguang ^{[1
,4
]}

Zheng, Xiaobo ^{[1
,4
]}

Gijzen, Mark ^{[6
]}

Wang, Yuanchao ^{[1
,4
]}

Dong, Suomeng ^{[1
,4
]}

机构：

[1] Nanjing Agr Univ, Dept Plant Pathol, Nanjing 210095, Jiangsu, Peoples R China

[2] Fujian Agr & Forestry Univ, Basic Forestry & Prote Res Ctr, Fujian Prov Key Lab Haixia Appl Plant Syst Biol, Fuzhou 350002, Fujian, Peoples R China

[3] Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Ctr Plant Stress Biol, Shanghai 201602, Peoples R China

[4] Minist Educ, Key Lab Integrated Management Crop Dis & Pests, Nanjing 210095, Jiangsu, Peoples R China

[5] South Univ Sci & Technol China, Dept Biol, Shenzhen 518055, Guangdong, Peoples R China

[6] Agr & Agri Food Canada, London, ON N5V 4T3, Canada

来源：

NATURE COMMUNICATIONS | 2017年 / 8卷

关键词：

NONSENSE-MEDIATED DECAY; ENDOPLASMIC-RETICULUM; BINDING PROTEIN; EFFECTOR; RESISTANCE; TRANSCRIPTION; ARABIDOPSIS; COMPLEXITY; INFECTION; TARGETS;

D O I：

10.1038/s41467-017-02233-5

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The process of RNA splicing influences many physiological processes, including plant immunity. However, how plant parasites manipulate host RNA splicing process remains unknown. Here we demonstrate that PsAvr3c, an avirulence effector from oomycete plant pathogen Phytophthora sojae, physically binds to and stabilizes soybean serine/lysine/arginine-rich proteins GmSKRPs. The SKRPs are novel proteins that associate with a complex that contains plant spliceosome components, and are negative regulators of plant immunity. Analysis by RNA-seq data indicates that alternative splicing of pre-mRNAs from 401 soybean genes, including defense-related genes, is altered in GmSKRP1 and PsAvr3c overexpressing lines compared to control plants. Representative splicing events mediated by GmSKRP1 and PsAvr3c are tested by infection assays or by transient expression in soybean plants. Our results show that plant pathogen effectors can reprogram host pre-mRNA splicing to promote disease, and we propose that pathogens evolved such strategies to defeat host immune systems.

引用

页数：15

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