Extraction and Purification of Lipoxygenase from Soybean Using Aqueous Two-Phase System

被引：21
作者：
Lakshmi, M. C. [1 ]
Madhusudhan, M. C. [1 ]
Raghavarao, K. S. M. S. [1 ]
机构：
[1] Cent Food Technol Res Inst, CSIR, Dept Food Engn, Mysore 570020, Karnataka, India
来源：
FOOD AND BIOPROCESS TECHNOLOGY | 2012年 / 5卷 / 01期
关键词：
Lipoxygenase; Aqueous two-phase extraction; Precipitation; Purification; LIQUID-LIQUID-EXTRACTION; PRECIPITATION; BROMELAIN;
D O I：
10.1007/s11947-009-0278-8
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The purification of lipoxygenase in soybeans (richest known source) was attempted by precipitation, using polyethylene glycol, followed by aqueous two-phase extraction (ATPE). The preliminary purification by precipitation was standardized which resulted in two-fold purification. The influence of process parameters such as phase forming salt, molecular weight of phase forming polymer, tie line length, and phase volume ratio on the partitioning behavior of lipoxygenase during ATPE was investigated. The aqueous two-phase system of polyethylene glycol 6000 and ammonium sulfate, with inherent pH of 5.2, was found to be most suitable. The tie line length of 41.25% and volume ratio of 1.7 have resulted in 125% activity recovery with the overall purification factor of 4.38 compared to crude enzyme extract.
引用
收藏
页码：193 / 199
页数：7
相关论文
共 29 条
[1]  
Agrawal Y. C., 2008, FOOD BIOPROCESS TECH, DOI [10.1007/s11947-008-0058-x, DOI 10.1007/S11947-008-0058-X]
[2]  
Albertsson P. A., 1986, PARTITION CELL PARTI, P303
[3]  
Axelrod B., 1981, METHODS ENZYMOLOGY, V71, P441, DOI [10.1016/0076-6879(81)71055-3, DOI 10.1016/0076-6879(81)71055-3]
[4]   Liquid-liquid extraction of bromelain and polyphenol oxidase using aqueous two-phase system [J].
Babu, B. Ravindra ;
Rastogi, N. K. ;
Raghavarao, K. S. M. S. .
CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION, 2008, 47 (01) :83-89
[5]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[6]   Aqueous two-phase systems extraction of Clostridium perfringens type α-toxin from A [J].
Cavalcanti, MTH ;
Porto, TS ;
Neto, BD ;
Lima-Filho, JL ;
Porto, ALF ;
Pessoa, A .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2006, 833 (02) :135-140
[7]   SEPARATION OF LIPOXYGENASE AND THE MAJOR SOYBEAN PROTEINS USING AQUEOUS 2-PHASE EXTRACTION AND POLY(ETHYLENE GLYCOL) PRECIPITATION SYSTEMS [J].
COLE, KD .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1993, 41 (02) :334-340
[8]   Extraction by reversed micelles of the intracellular enzyme xylose reductase [J].
Cortez, EV ;
Felipe, MDD ;
Roberto, IC ;
Pessoa, A ;
Vitolo, M .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2001, 91-3 (1-9) :753-759
[9]  
Diamond A D, 1992, Adv Biochem Eng Biotechnol, V47, P89
[10]  
Fox B.G., 1998, Comprehensive Biological Catalysis, VIII, P262
123