DNA binding studies of cationic lanthanide complexes bearing a phenanthridinium group

被引：29

作者：

Bobba, G ^{[1
]}

Kean, SD ^{[1
]}

Parker, D ^{[1
]}

Beeby, A ^{[1
]}

Baker, G ^{[1
]}

机构：

[1] Univ Durham, Dept Chem, Durham DH1 3LE, England

来源：

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2 | 2001年 / 09期

关键词：

D O I：

10.1039/b104795g

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The binding of tetracationic Lambda- and Delta -europium complexes of tetraamide and triamide ligands incorporating an N-methylphenanthridinium group to calf-thymus DNA, poly(dGdC) and super-coiled plasmid DNA has been monitored by absorption, luminescence and CD difference spectroscopy. With the tetraamide complexes, preferential binding to (CG) base-pairs was signalled by pronounced hypochromism, a small red shift and substantial fluorescence quenching with a limiting base-pair complex ratio of 2:1, consistent with an intercalative interaction and charge neutralisation. For the triamide Eu complex; a lower base-pair : complex ratio was found and changes in the form and polarisation of the Eu emission spectrum were consistent with coordination of a nucleobase phosphate group to the hydrated Eu centre.

引用

收藏

页码：1738 / 1741

页数：4

相关论文

共 27 条

[1] QUANTITATIVE DETERMINATION OF MONONUCLEOTIDE CONFORMATIONS IN SOLUTION USING LANTHANIDE ION SHIFT AND BROADENING NMR PROBES [J].

BARRY, CD ;

NORTH, ACT ;

GLASEL, JA ;

WILLIAMS, RJP ;

XAVIER, AV .

NATURE, 1971, 232 (5308) :236-+

[2] BINDING MODES AND BASE SPECIFICITY OF TRIS(PHENANTHROLINE)RUTHENIUM(II) ENANTIOMERS WITH NUCLEIC-ACIDS - TUNING THE STEREOSELECTIVITY [J].

BARTON, JK ;

GOLDBERG, JM ;

KUMAR, CV ;

TURRO, NJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1986, 108 (08) :2081-2088

[3]

Beeby A, 2000, PHOTOCHEM PHOTOBIOL, V72, P10, DOI 10.1562/0031-8655(2000)072<0010:TPPOMA>2.0.CO

[4]

2

[5]

BOBBA G, 2001, J CHEM SOC P2

[6] High-yield preparation of oligomeric C-type DNA toroids and their characterization by cryoelectron microscopy [J].

Böttcher, C ;

Endisch, C ;

Fuhrhop, JH ;

Catterall, C ;

Eaton, M .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (01) :12-17

[7] The selectivity of reversible oxy-anion binding in aqueous solution at a chiral europium and terbium center: Signaling of carbonate chelation by changes in the form and circular polarization of luminescence emission [J].

Bruce, JI ;

Dickins, RS ;

Govenlock, LJ ;

Gunnlaugsson, T ;

Lopinski, S ;

Lowe, MP ;

Parker, D ;

Peacock, RD ;

Perry, JJB ;

Aime, S ;

Botta, M .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (40) :9674-9684

[8] Effect of mixed pendent groups on the solution and catalytic properties of europium(III) macrocyclic complexes: Bifunctional and monofunctional amide and alcohol pendents in septadentate or octadentate ligands [J].

Chappell, LL ;

Voss, DA ;

Horrocks, WD ;

Morrow, JR .

INORGANIC CHEMISTRY, 1998, 37 (16) :3989-3998

[9] Experimental assessment of the efficacy of sensitised emission in water from a europium ion, following intramolecular excitation by a phenanthridinyl group [J].

Clarkson, IM ;

Beeby, A ;

Bruce, JI ;

Govenlock, LJ ;

Lowe, MP ;

Mathieu, CE ;

Parker, D ;

Senanayake, K .

NEW JOURNAL OF CHEMISTRY, 2000, 24 (06) :377-386

[10] Eu(III) macrocyclic complexes promote cleavage of and bind to models for the 5′-cap of mRNA.: Effect of pendent group and a second metal ion [J].

Epstein, DM ;

Chappell, LL ;

Khalili, H ;

Supkowski, RM ;

Horrocks, WD ;

Morrow, JR .

INORGANIC CHEMISTRY, 2000, 39 (10) :2130-2134