A cost-effective RNA sequencing protocol for large-scale gene expression studies

被引：72

作者：

Hou, Zhonggang ^{[1
]}

Jiang, Peng ^{[1
]}

Swanson, Scott A. ^{[1
]}

Elwell, Angela L. ^{[1
]}

Nguyen, Bao Kim S. ^{[1
]}

Bolin, Jennifer M. ^{[1
]}

Stewart, Ron ^{[1
]}

Thomson, James A. ^{[1
,2
,3
]}

机构：

[1] Morgridge Inst Res, Madison, WI 53715 USA

[2] Univ Wisconsin, Dept Cell & Regenerat Biol, Madison, WI USA

[3] Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

美国国家卫生研究院;

关键词：

SEQ;

D O I：

10.1038/srep09570

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

RNA sequencing has increasingly become an indispensable tool for biological research. While sequencing costs have fallen dramatically in recent years, the current cost of RNA sequencing, nonetheless, remains a barrier to even more widespread adoption. Here, we present a simple RNA sequencing protocol with substantially reduced costs. This protocol uses as little as 10 ng of total RNA, allows multiplex sequencing of up to 96 samples per lane, and is strand specific. Extensive validation using human embryonic stem cells showed high consistency between technical replicates at various multiplexing levels.

引用

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